Structure of PDB 7y5n Chain C

Receptor sequence

>7y5nC (length=1115) Species: 9606 (Homo sapiens)

PSRALYFSGRGEQLRLRADLELPRDAFTLQVWLRAEGGQRSPAVITGLYD
KCSYISRDRGWVVGIHTISDQDNKDPRYFFSLKTDRARQVTTINAHRSYL
PGQWVYLAATYDGQFMKLYVNGAQVATSGEQVGGIFSPLTQKCKVLMLGG
SALNHNYRGYIEHFSLWKVARTQREILSDMETHGAHTALPQLLLQENWDN
VKHAWSPMKDGSSPKVEFSNAHGFLLDTSLEPPLCGQTLCDNTEVIASYN
QLSSFRQPKVVRYRVVNLYEDDHKNPTVTREQVDFQHHQLAEAFKQYNIS
WELDVLEVSNSSLRRRLILANCDISKIGDENCDPECNHTLTGHDGGDCRH
LRHPAFVKKQHNGVCDMDCNYERFNFDGGECCDPEITNVTQTCFDPDSPH
RAYLDVNELKNILKLDGSTHLNIFFAKSSEEELAGVATWPWDKEALMHLG
GIVLNPSFYGMPGHTHTMIHEIGHSLGLYHVFRGISEIQSCSDPCMETEP
SFETGDLCNDTNPAPKHKSCGDPGPGNDTCGFHSFFNTPYNNFMSYADDD
CTDSFTPNQVARMHCYLDLVYQGWQPSRKPAPVALAPQVLGHTTDSVTLE
WFPPIDGHFFERELGSACHLCLEGRILVQYASNASSPMPCSPSGHWSPRE
AEGHPDVEQPCKSSVRTWSPNSAVNPHTVPPACPEPQGCYLELEFLYPLV
PESLTIWVTFVSTDWDSSGAVNDIKLLAVSGKNISLGPQNVFCDVPLTIR
LWDVGEEVYGIQIYTLDEHLEIDAAMLTSTADTPLCLQCKPLKYKVVRDP
PLQMDVASILHLNRKFVDMDLNLGSVYQYWVITISGTEESEPSPAVTYIH
GSGYCGDGIIQKDQGEQCDDMNKINGDGCSLFCRQEVSFNCIDEPSRCYF
HDGDGVCEEFEQKTSIKDCGVYTPQGFLDQWASNASVSHQDQQCPGWVII
GQPAASQVCRTKVIDLSEGISQHAWYPCTISYPYSQLAQTTFWLRAYFSQ
PMVAAAVIVHLVTDGTYYGDQKQETISVQLLDTKDQSHDLGLHVLSCRNN
PLIIPVVHDLSQPFYHSQAVRVSFSSPLVAISGVALRSFDNFDPVTLSSC
QRGETYSPAEQSCVH

3D structure

• PDB: 7y5n Structural insights into the covalent regulation of PAPP-A activity by proMBP and STC2.
• Chain: C
• Resolution: 3.45 Å

Enzymatic activity

• Enzyme Commision number: 3.4.24.79: pappalysin-1.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	C	H482 H486 H492	H470 H474 H480
BS02	CA	C	A663 D668 R678 D785	A651 D656 R666 D773
BS03	CA	C	N374 V376 D378 D389 E392	N362 V364 D366 D377 E380
BS04	CA	C	Y866 D869 I871 Q873 E878 D881	Y854 D857 I859 Q861 E866 D869
BS05	CA	C	E509 D518 C520 T523	E497 D506 C508 T511
BS06	CA	C	H913 D916 V918 E923 D930	H901 D904 V906 E911 D918
BS07	CA	C	K338 D341 N343 D345 D356	K326 D329 N331 D333 D344
BS08	CA	C	N884 I886 C891	N872 I874 C879

Gene Ontology

Molecular Function

• GO:0004175 endopeptidase activity
• GO:0004222 metalloendopeptidase activity
• GO:0005515 protein binding
• GO:0008237 metallopeptidase activity
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006508 proteolysis
• GO:0007166 cell surface receptor signaling pathway
• GO:0007565 female pregnancy
• GO:0030163 protein catabolic process
• GO:0032354 response to follicle-stimulating hormone
• GO:0071548 response to dexamethasone

Cellular Component

• GO:0005576 extracellular region
• GO:0005615 extracellular space

External links

• PDB: RCSB:7y5n, PDBe:7y5n, PDBj:7y5n
• PDBsum: 7y5n
• PubMed: 36550107
• UniProt: Q13219|PAPP1_HUMAN Pappalysin-1 (Gene Name=PAPPA)