Structure of PDB 7xyb Chain C

Receptor sequence

>7xybC (length=1190) Species: 287 (Pseudomonas aeruginosa)

YSYTEKKRIRKDFSKLPDVMDVPYLLAIQLDSYREFLQAGATKEQFRDVG
LHAAFKSVFPIISYSGNAALEYVGYRLGEPAFDVKECVLRGVTFAVPLRV
KVRLIIFDRESSNKAIKDIKEQEVYMGEIPLMTENGTFIINGTERVIVSQ
LHRSPGVFFDHDRGKTHSSGKLLYSARIIPYRGSWLDFEFDPKDCVFVRI
DRRRKLPASVLLRALGYSTEEILNAFYATLYTNDIDCGPFISDTLKIDNT
SNQLEALVEIYRMMRPGEPPTKEAAETLFGNLFFSAERYDLSAVGRMKFN
RRIGRTEIEGPGVLSKEDIIDVLKTLVDIRNGKGIVDDIDHLGNRRVRCV
GEMAENQFRVGLVRVERAVKERLSMAESEGLMPQDLINAKPVAAAIKEFF
GSSQLSQFMDQNNPLSEITHKRRVSALGPGGLTRERAGFEVRDVHPTHYG
RVCPIETPEGPNIGLINSLATYARTNKYGFLESPYRVVKDSLVTDEIVFL
SAIEEADHVIAQASATLNEKGQLVDELVAVRHLNEFTVKAPEDVTLMDVS
PKQVVSVAASLIPFLEHDDANRALMGSNMQRQAVPTLRADKPLVGTGMER
NVARDSGVCVVARRGGVIDSVDASRVVVRVADDEVETGEAGVDIYNLTKY
TRSNQNTCINQRPLVSKGDVVARGDILADGPSTDMGELALGQNMRVAFMP
WNGFNFEDSICLSERVVQEDRFTTIHIQELTCVARDTKLGPEEITADIPN
VGEAALNKLDEAGIVYVGAEVQAGDILVGKVTPVKDTSLRVPTGTKGTVI
DVQVFTRDGVERDSRALSIEKMQLDQIRKDLNEEFRIVEGATFERLRAAL
VGAADDALNEQLEKAQAYISDRRQLLDDKFEDKKRKLQQGDDLAPGVLKI
VKVYLAIKRRIQPGDKMAGRHGNKGVVSVIMPVEDMPHDANGTPVDIVLN
PLGVPSRMNVGQILETHLGLAAKGLGEKINRMLEEQRKVAELRKFLHEIY
NEIGGREENLDELGDNEILALAKNLRGGVPMATPVFDGAKEREIKAMLKL
ADLPESGQMRLFDGRTGNQFERPTTVGYMYMLKLNHLVDDKMHARSTGSY
SLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGR
TKMYKNIVDGDHRMEAGMPESFNVLIKEIRSLGIDIELET

3D structure

• PDB: 7xyb Structural basis of AlpA-dependent transcription antitermination.
• Chain: C
• Resolution: 3.7 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	C	Q515 Q518 R545 P569 N573 R692 Q693 K1090 H1252	Q404 Q407 R434 P458 N462 R581 Q582 K924 H1086
BS02	dna	C	G185 R547	G183 R436
BS03	dna	C	R147 K508 K1277	R145 K397 K1111

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0032549 ribonucleoside binding
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex

External links

• PDB: RCSB:7xyb, PDBe:7xyb, PDBj:7xyb
• PDBsum: 7xyb
• PubMed: 35871295
• UniProt: Q51561|RPOB_PSEAE DNA-directed RNA polymerase subunit beta (Gene Name=rpoB)