Structure of PDB 7uix Chain C

Receptor sequence

>7uixC (length=580) Species: 562 (Escherichia coli)

ENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGVG
KTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKA
LLKQLEQDTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVI
GSTTYQEFSNIFEKDRALARRFQKIDITEPSIEETVQIINGLKPKYEAHH
DVRYTAKAVRAAVELAVKYINDRHLPDKAIDVIDEAGARARLMPVSKRKK
TVNVADIESVVARIARIPEKSVSQSDRDTLKNLGDRLKMLVFGQDKAIEA
LTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQLSKALGIELLRF
DMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEIEKA
HPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGL
IHQDNSTDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVEL
QVQLDQKGVSLEVSQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANE
LLFGSLVDGGQVTVALDKEKNELTYGFQSA

3D structure

• PDB: 7uix AAA+ protease-adaptor structures reveal altered conformations and ring specialization.
• Chain: C
• Resolution: 3.24 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	C	K258 H528 G539 Y540 V541	K89 H359 G370 Y371 V372
BS02	AGS	C	L188 I189 G219 K220 T221 A222 P395 I399	L19 I20 G50 K51 T52 A53 P226 I230
BS03	AGS	C	T497 G498 V499 G500 T502 E503 E565 N606 V661 K664 R702	T328 G329 V330 G331 T333 E334 E396 N437 V492 K495 R533
BS04	MG	C	T502 D564	T333 D395

Gene Ontology

Molecular Function

• GO:0004176 ATP-dependent peptidase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity

Biological Process

• GO:0006508 proteolysis
• GO:0006515 protein quality control for misfolded or incompletely synthesized proteins
• GO:0006979 response to oxidative stress
• GO:0034605 cellular response to heat
• GO:0043335 protein unfolding

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0009368 endopeptidase Clp complex

External links

• PDB: RCSB:7uix, PDBe:7uix, PDBj:7uix
• PDBsum: 7uix
• PubMed: 36329286
• UniProt: P0ABH9|CLPA_ECOLI ATP-dependent Clp protease ATP-binding subunit ClpA (Gene Name=clpA)