Structure of PDB 7tfi Chain C

Receptor sequence
>7tfiC (length=330) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]
EKRSKENLPWVEKYRPETLDEVYGQNEVITTVRKFVDEGKLPHLLFYGPP
GTGKTSTIVALAREIYGKNYSNMVLELNASDDRGIDVVRNQIKDFASTRQ
IFSKGFKLIILDEADAMTNAAQNALRRVIERYTKNTRFCVLANYAHKLTP
ALLSRCTRFRFQPLPQEAIERRIANVLVHEKLKLSPNAEKALIELSNGDM
RRVLNVLQSCKATLDNPDEDEISDDVIYECCGAPRPSDLKAVLKSILEDD
WGTAHYTLNKVRSAKGLALIDLIEGIVKILEDYELQNEETRVHLLTKLAD
IEYSISKGGNDQIQGSAVIGAIKASFENET
3D structure
PDB7tfi Cryo-EM structures reveal that RFC recognizes both the 3'- and 5'-DNA ends to load PCNA onto gaps for DNA repair.
ChainC
Resolution3.41 Å
3D
structure
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Enzymatic activity
Enzyme Commision number ?
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna C G89 I90 R94 G84 I85 R89
BS02 AGS C Y19 R20 Y28 G56 T57 G58 K59 T60 S61 N148 R177 M205 R206 Y14 R15 Y23 G51 T52 G53 K54 T55 S56 N143 R172 M200 R201
BS03 MG C T60 D117 T55 D112
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003689 DNA clamp loader activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016887 ATP hydrolysis activity
Biological Process
GO:0000077 DNA damage checkpoint signaling
GO:0006260 DNA replication
GO:0006261 DNA-templated DNA replication
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006272 leading strand elongation
GO:0006281 DNA repair
GO:0006298 mismatch repair
GO:0007064 mitotic sister chromatid cohesion
GO:0090618 DNA clamp unloading
Cellular Component
GO:0005634 nucleus
GO:0005663 DNA replication factor C complex
GO:0005829 cytosol
GO:0031389 Rad17 RFC-like complex
GO:0031390 Ctf18 RFC-like complex
GO:0031391 Elg1 RFC-like complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:7tfi, PDBe:7tfi, PDBj:7tfi
PDBsum7tfi
PubMed35829698
UniProtP38629|RFC3_YEAST Replication factor C subunit 3 (Gene Name=RFC3)

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