Structure of PDB 7t3t Chain C

Receptor sequence

>7t3tC (length=2121) Species: 9606 (Homo sapiens)

SSFLHIGDIVSLYAEGSVNGFISTLGLVDDRCVVEPAAGDLDNPPKKFRD
CLFKVCPMNRYSAQKQYWKAKQVVLLQKLQHAAQMEQKQNDTENKKVHGD
VVKYGSVIQLLHMKSNKYLTVNKRLPALLEKNAMRVTLDATGNEGSWLFI
QPFWKLRSNGDNVVVGDKVILNPVNAGQPLHASNYELSDNAGCKEVNSVN
CNTSWKINLFMQFRDHLEEVLKGGDVVRLFHAEQEKFLTCDEYKGKLQVF
LRTTLRQSATSATSSNALWEVEVVHHDPCRGGAGHWNGLYRFKHLATGNY
LAAEENPSYKIKYCLVAVPHGNDIASLFELDPTTLQKTDSFVPRNSYVRL
RHLCTNTWIQSTNVPIDIEEERPIRLMLGTCPTKEDKEAFAIVSVPVSEI
RDLDFANDASSMLASAVEKLNEGFISQNDRRFVIQLLEDLVFFVSDVPNN
GQNVLDIMVTKPNRERQKLMREQNILKQVFGILKAPFRPLVRLEELSDQK
NAPYQHMFRLCYRVLRHSQEDYRKNQEHIAKQFGMMQSQIGYDILAEDTI
TALLHNNRKLLEKHITKTEVETFVSLVRKNREPRFLDYLSDLCVSNHIAI
PVTQELICKCVLDPKNSDILIRTELRPEVWLTWTDKNNEHHEKSVRQLAQ
EARAGNAHDENVLSYYRYQLKLFARMCLDRQYLAIDEISQQLGVDLIFLC
MADEMLPFDLRASFCHLMLHVHVDRDPQELVTPVKFARLWTEIPTAITIK
DYDSNLNNKFANTMEFVEDYLNNVVSEAVPFANEEKNKLTFEVVSLAHNL
IYFGFYSFSELLRLTRTLLGIIDDIVVMETKLKILEILQFILNVRLDYRI
SYLLSVFKKEFLDRIGEQAEAMFGLEVDDEGGRMFLRVLIHLTMHDYAPL
VSGALQLLFKHFSQRQEAMHTFKQVQLLISAQDVENYKVIKSELDRLRTM
VEKSELWNYQIVKGILERLNKMCGVGEQMRKKQQRLLKNMDAHKVMLDLL
QIPYDKGDAKMMEILRYTHQFLQKFCAGNPGNQALLHKHLHLFLTPGLLE
AETMQHIFLNNYQLCSEISEPVLQHFVHLLATHGRHVQYLDFLHTVIKAE
GKYVKKCQDMIMTELTNAGDDVVVFYNDKASLAHLLDMMKAARDGVEDHS
PLMYHISLVDLLAACAEGKNVYTEIKCTSLLPLEDVVSVVTHEDCITEVK
MAYVNFVNHCYVDTEVEMKEIYTSNHIWTLFENFTLDMARVPTLEKYVLS
VVLDTINAFFSSPFSENSTTHQTIVVQLLQSTTRLGSVEACIRTLAMVAK
GRWDYKNIIEKLQDIITALEERLKPLVQAELSVLVDVLHWPELLFLEGSE
AYQRCESGGFLSKLIQHTKDLMESEEKLCIKVLRTLQQMLLKKTGNQLRK
MLLQNYLQNSAIAATQCRLDKEGATKLVCDLITSTKNEKIFQESIGLAIH
LLDGGNTEIQKSFHNLMMSDKKSERFFKVLHDRMKRAQQETKSTGTSVLI
MQPILRFLQLLCENHNRDLQNFLRCQNNKTNYNLVCETLQFLDIMCGSTT
GGLGLLGLYINEDNVGLVIQTLETLTEYCQGPCHENQTCIVTHESNGIDI
ITALILNDISPLCKYRMDLVLQLKDNASKLLLALMESRHDSENAERILIS
LRPQELVDVIKKAYLQSEVSPREVGHNIYILALQLSRHNKQLQHLLKPDP
LAYYENHTSQIEIVRQDRSMEQIVFPVPGICQFLTEETKHRLFTTTEQDE
QGSKVSDFFDQSSFLHNEMEWQRKLRSMPLIYWFSRRMTLWGSISFNLAV
FINIIIAFFYPYIVALILRSIYYLGIGPTLNILGALNLTNKIVFVVSFVG
NRGTFMEFLYHVGYILTSVLGLFAHELFYSILLFDLIYREETLFNVIKSV
TRNGRSILLTALLALILVYLFSIVGFLFLKDDFILEVDRLSTERACDTLL
MCIVTVMNHGLRNGGGVGDILRKPSKDESLFPARVVYDLLFFFIVIIIVL
NLIFGVIIDTFADLRSEKQKKEEILKTTCFICGLERDKFDNKTVSFEEHI
KLEHNMWNYLYFIVLVRVKNKTDYTGPESYVAQMIKNKNLDWFPRMRAMS
LVAAAAAAAAAAAAAAAAAAA

3D structure

• PDB: 7t3t Structural basis for activation and gating of IP 3 receptors.
• Chain: C
• Resolution: 3.8 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	C	C2538 H2558 H2563	C2029 H2049 H2054
BS02	I3P	C	R266 T268 L269 R270 K507 R510 Y567 R568 K569	R252 T254 L255 R256 K468 R471 Y522 R523 K524
BS03	ATP	C	K2152 C2538 F2539 I2559 K2560 N2564 M2565 W2566	K1739 C2029 F2030 I2050 K2051 N2055 M2056 W2057
BS04	CA	C	E1882 E1946 T2581	E1513 E1577 T2072

Gene Ontology

Molecular Function

• GO:0000822 inositol hexakisphosphate binding
• GO:0005216 monoatomic ion channel activity
• GO:0005220 inositol 1,4,5-trisphosphate-gated calcium channel activity
• GO:0005262 calcium channel activity
• GO:0005509 calcium ion binding
• GO:0005515 protein binding
• GO:0015278 intracellularly gated calcium channel activity
• GO:0035091 phosphatidylinositol binding
• GO:0043533 inositol 1,3,4,5 tetrakisphosphate binding
• GO:0070679 inositol 1,4,5 trisphosphate binding

Biological Process

• GO:0006811 monoatomic ion transport
• GO:0006816 calcium ion transport
• GO:0007186 G protein-coupled receptor signaling pathway
• GO:0007204 positive regulation of cytosolic calcium ion concentration
• GO:0007613 memory
• GO:0019722 calcium-mediated signaling
• GO:0030168 platelet activation
• GO:0050909 sensory perception of taste
• GO:0050913 sensory perception of bitter taste
• GO:0050916 sensory perception of sweet taste
• GO:0050917 sensory perception of umami taste
• GO:0051209 release of sequestered calcium ion into cytosol
• GO:0051289 protein homotetramerization
• GO:0051592 response to calcium ion
• GO:0055074 calcium ion homeostasis
• GO:0055085 transmembrane transport
• GO:0060291 long-term synaptic potentiation
• GO:0070588 calcium ion transmembrane transport

Cellular Component

• GO:0005634 nucleus
• GO:0005640 nuclear outer membrane
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005789 endoplasmic reticulum membrane
• GO:0005886 plasma membrane
• GO:0005903 brush border
• GO:0016020 membrane
• GO:0016529 sarcoplasmic reticulum
• GO:0030658 transport vesicle membrane
• GO:0030667 secretory granule membrane
• GO:0031095 platelet dense tubular network membrane
• GO:0031410 cytoplasmic vesicle
• GO:0043025 neuronal cell body
• GO:0043235 receptor complex
• GO:0045177 apical part of cell
• GO:0098554 cytoplasmic side of endoplasmic reticulum membrane

External links

• PDB: RCSB:7t3t, PDBe:7t3t, PDBj:7t3t
• PDBsum: 7t3t
• PubMed: 35301323
• UniProt: Q14573|ITPR3_HUMAN Inositol 1,4,5-trisphosphate-gated calcium channel ITPR3 (Gene Name=ITPR3)