Structure of PDB 7moq Chain C

Receptor sequence

>7moqC (length=4159) Species: 452467 (Tetrahymena thermophila CU428)

SKRIAWMKTTICDSLQLKDMIVEESFQYEKNKNLLEQFLSGEGLNKIFAY
YQVQEQAQNDDIKDTGAQDPVLFFTTGDLEKIQDKAVWFLRITNPADDKK
KASQQDGNDNDIIFGEITPNTVPMLNALMESVYSRQIDHIITEKIQFWGV
AEEEQVLEFQQHSNKFSSEVREANLMSPGTEHFKLDYEAISGLSESEKMQ
HYEMKFNEWINLISSQLNRKDEKDAGPATELIYWRSRMQKITNWSEQLKS
KDFQIVKASLQRHKNHDNQRPRGDESLNEAKDNVKYLTTLEKFIEPLYNG
TPQQIIDTLPALMNAIKMIHTIARFYNTTDKMTGLFIKITNQMIKNCKDR
ILNMIWEQDPAELIEVLGSCIKLYCEYKKCYNDTKEKVADMPKGKTFDFS
DAQIFGKFDTFVRRLQKLIEIFSNIQQFNALAKHNLEGMDVLTNKFKKII
DDFKKKGHNLLDTANNKFDRDWVEFNVEISHLDGELQNFIDNNFNRFRNI
EYSLKLLHKFQSTIKRDSLKHNLTSRYNAILHNYATELDTIQRVFQDQKS
NPPLVRNMPPEAGKIIWARHLFQKITGPINIFPENVINSTEIRRYYGSYN
TLGKQLTIYEMWFYQDWVNKIEQSKAALQATLIVRHDENKKLYVNFDLEI
MQLIREAKCLDRQGIEIPESARIILLQEDKFKTYYNELLYALKEYERINS
KIKPICKNLLLPHIEDLDLKLRPGMVTLTWTSMNIESYLYYVHQGLKKLE
QLIINVNDIIENRIENNLKTVSKVVLVHLPNVEVERAVDDLLQTIMLYPL
DPHVDPVLPEETKRIKRYYFWYFYQALLNSTQNSLNAMKYRVCGKKIPGA
NTLQNLKPFFQVEVQLNGDKVTLNPSLQEIQKSINRAATAVLRCSKHLYN
WDQQNKDSTDKATFYDMIACDKEIVKVILLLTGSIQGTKNKVNEFLSGFT
KFEWLCKESIQESIKKFSKNGPTLQNYEDQLKKFSQIEEEIEKIVPTYKI
GAMELMTHNICTSLSTWAKEWKLQYSQDLHKRARQLLDSLTEQTKMLSTK
LSKPVKDIDSLGYVMETLEQIRKEQAEIDMKFNPVQEMYSLLDNYLPGGI
TDKDEMDARSLLRRNWDILIQQAEIKGKEYQHKQAIYLKELKQSIKDFTN
QVSIFRRDYEKNGPMVEGISPAEAMERLRRFEDEYDVKYQMYKINARGEN
LFGLQNQKYPELEKTDAEIKNLNKLYNLYDSVIKNIQQFKEKSWQDVSKD
DLAKMEEDAGKYGEQCSRLPKDLKEWQAYRDLKNYIDSLREQLPLIISLK
KPSIMPRHWEKIKEITNTKLNYENPDQFYIEEIMGAKLLDFREDIEDITE
SADKQLKIRTGLDEINLYWNDMQFQFGIWGKRDVPCMLNGLIVGTILERL
EEDQLQLSTFNSQRHVTPFKAEVENLIRTFSDVNDTLDMWVKVQKLWTSL
EPVFTGGDIARQMPLQAKQFQGIDKNWMKIMEKAVETKKVIPCCQNDMLK
DFLPDLNRKLEDCQKMLEAYLEGKRKKFPRFYFVSNPTLLKILSQGSEPT
SIQEDFEKLFDAITKVTFESAKDKKNPALKQITQIQQVIGRNEENISLTG
YYVKCEGNIEDWLKKLEQNMQQTLKDIASAAAQQVFQVGLKEFVSSQASQ
IALLGLQILWTSKVNEGLERLSRNERNAMDIKRNEIKEHMNILSSMCLED
LNGAVERTKVETLVTIQVHQKDISMDLKCKDVNDFEWQKQTRIAWKTDID
ECIISITDWDSPYSYEFLGAKERLCITPLTDRCYITLAQAMSMYYGGAPA
GPAGTGKTETVKDLGRTLGVFVVVTNCSDQHRYRDMAKIFKGLVQSGLWG
CFDEFNRIDLEVLSVVAMQVESITTARKQHMKKFMFPEEEIEIELIPTVS
YFITMNPGYAGRQELPENLKVLFRGVSMMVPDREIIIKVKLASVGYLQID
LLAKKFNVLYRLCEEQLSKQRHYDFGLRNILSVLRTAGNTKRQEIKSDEE
MLLMRSLRDMNLSKLVADDIPLFNGLLADIFPKLKEVPKKLYPDVEKKIP
EEINAESYLINTPSFQLKIIQLYETCLVRHGFMLVGPTGSGKSTIMKILT
EVLTKLGSPHKIVIMNPKAITAEQMYGVKSEISDDWIPGVFSTIWAKSNN
RALKHTTWITCDGPVDAIWIENLNTVLDDNKILTLANGERIAMTENCKVV
FEVENLNNASPATVSRCGQVYVSPTDLGYEAVIEGWIRNRKASGRAEESD
KLGNILRKYLINMRFIELQSKECKEPMMDTSPVISVINILNLLTGCLQYF
VQTQRTLSEQEYEKFIVYSMAWAIGGIYEAQDRVRFHELLLAKNAPIPQK
GKENETVFDYYVSQDYLDWKICSPEEWVPPQSLQFSQLLLPTLDSFRAEM
LLNFILTQPKSHTCSNSALLIGGSGTAKTSSVLLYCNKFDPQKMLFKRTN
FSSATSPFMFQSTIEAECDFKVGKEFAPPGNKMMTIFIDDMSMPFVNKWG
DQITLELVRQLIETGGFYMLDKTQRGNQRKMKNLQYIGAMNHPGGGRNDI
PNRLKRQFFIFNMILPLSIEGIYGPIIKHMFKQKYFSDSTYKVIESLTSA
TIALWNKVKSTMLPTPAKFHYVFNMRELSRIFKGILTCKKDTINDAPKSM
KIKPELFLVGLWRHEAERVLADKLVNNKDKDTVMGYIQEVSLESFSQIEN
EILEKYSSEKTFLFCDFLRPDVINEDGIIEEEAPKIYEAIDSLTELRKRC
NFLLSFYNDRNPSKKMPLVLFDDALKHLLRISRIIRQPRSSGLLVGVGGS
GKQSLTRLAGFIGKNLIQQIIVTKTYSDKDLKEDIKKGFDDAGHLGKQVT
FLMTDSEVKKEEFLEYINMVLSTGEIPNLLAKDEREVWLGDISQAYCKEK
NLGNIDPPQSELWTYFVDRVRDNFHIMLCFSPVGQKFRERARKFPALFNE
CTIDWFLPWPEEALVSVAETFIKNFDKLDTKEETKQELMKHMGNVHLMVN
EICDEYYQKMRRQVYVTPKSFLSYLNSYKTLYIEKYDELDQQEESFKIGL
NKIQGEKDRWGKGAQDISDQKRKLVGNVSLSTAFISYCGPFNAEYRNKLA
QQRFVVDMKKRGVPVTPGLELTSFLIDDATIGEWNLQGLPKDDLSIQNGI
MVTNSARYPLFIDPQGQGQNWIRNKLSAITTLSHPKFKDMFLKYCMESGL
TLIVENIENEVDPMMDPVLELSKEFKLFMTCRLANPSFSPELSAKTTIID
FTVTQSGLEQQLLGKVISKEQKALEDSLNQLLADVNQNQKDLQRLDKNLL
ERLINSQGNLLDDTELMDVLNNTKTQAKEVAAKLIDAEIKTKEINEKREQ
YRPVAIRGSAIYFTMIEVSLVNWMYNSSLEQFLKLFIESIDLSEKAQLPS
NRVKNIISFLTFHVYRYVNRGLFEKDKITFILMMAFKILTTAGTISSGDV
SLFLKSQKQISYLEDNQWLNILALSKPFFKELPDLISRSENQWRNWIDKN
DPENFPIPDFAESINQEKEIGSFISLCLVRSLRNDRTLIATQNFISNVLG
KEFTDPISYPIEGIWQESSNMDPVLFLLSAGADPTSSIDELAKKKKKFPC
EKVSMGEGQERVARQVIMKGFVEGGWVILQNCHLGLKFMEEIETLVSPIN
QIHEDFRLWITCEQHPKFPLGLLQKTLKVTNEPPKGLKAGLYKTFTTIIT
QEFIDKVDHSNWRSLIFTICFLHSIVIERKKFGPLGWCVPYEYNYSDLEA
SLLYIEKYLTNLMSTPQPNSHNLPISMNVVRYMICEVQYGGRITDDLDRE
LFITYGETYLKDGIFGNDYFFPQNPSAELIKYQEYIAKVPTVDNPEVFGL
HSNADLTFRLKESKEMINTVMETRPREEIVQDKAKDMLKNLPPDYNDVEV
RELVSKLGGPNPKTSTERGMTVPLNIFLYQEVTRMQRVIGLVRKTLQDTI
LAIDGQIIMTPEILEAINAIYDAKVPNSWLYDPSGAEISWLLPNLGSWST
SLSDRNKQLNDWLRSGQRPILFWLTGFFNPQGFLTGMKQEVTRNHKKEAW
SLDDVVYSTTVKEREKEPAEGVYIKGLYLEGCKWSKNGLDDSDPKKIFAD
LPILHVSAINKSNTYLCPVYKYPKRTDKYLIFRVGLPCEGSNNPSHWKLR
GVALLCSTE

3D structure

• PDB: 7moq Remodeling and activation mechanisms of outer arm dyneins revealed by cryo-EM.
• Chain: C
• Resolution: 8.0 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	C	A1871 G1872 P1873 A1874 T1876 G1877 K1878 E1925 N1977 P1978 G1979 Y1980 R1983 M1999	A1800 G1801 P1802 A1803 T1805 G1806 K1807 E1854 N1906 P1907 G1908 Y1909 R1912 M1928
BS02	ATP	C	T2526 S2527 P2528 F2566 I2574 T2575 E2577 L2578	T2455 S2456 P2457 F2495 I2503 T2504 E2506 L2507
BS03	ADP	C	L2849 I2852 G2872 K2873 Q2874 S2875 L2876 T2877 L2879 A2880 G2881	L2778 I2781 G2801 K2802 Q2803 S2804 L2805 T2806 L2808 A2809 G2810

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008569 minus-end-directed microtubule motor activity
• GO:0016887 ATP hydrolysis activity
• GO:0045505 dynein intermediate chain binding
• GO:0051959 dynein light intermediate chain binding

Biological Process

• GO:0007018 microtubule-based movement

Cellular Component

• GO:0005737 cytoplasm
• GO:0005856 cytoskeleton
• GO:0005858 axonemal dynein complex
• GO:0005874 microtubule
• GO:0005929 cilium
• GO:0030286 dynein complex

External links

• PDB: RCSB:7moq, PDBe:7moq, PDBj:7moq
• PDBsum: 7moq
• PubMed: 34338432
• UniProt: Q22A67