Structure of PDB 7mlj Chain C

Receptor sequence

>7mljC (length=1111) Species: 300852 (Thermus thermophilus HB8)

MEIKRFGRIREVIPLPPLTEIQVESYRRALQADVPPEKRENVGIQAAFRE
TFPIEELVLDFLEYRLGEPPFPQDECREKDLTYQAPLYARLQLIHKDTGL
IKEDEVFLGHIPLMTEDGSFIINGADRVIVSQIHRSPGVYFTPDPARPGR
YIASIIPLPKRGPWIDLEVEPNGVVSMKVNKRKFPLVLLLRVLGYDQETL
ARELGAYGELVQGLMDESVFAMRPEEALIRLFTLLRPGDPPKRDKAVAYV
YGLIADPRRYDLGEAGRYKAEEKLGIRLSGRTLARFEDGEFKDEVFLPTL
RYLFALTAGVPGHEVDDIDHLGNRRIRTVGELMTDQFRVGLARLARGVRE
RMLMGSEDSLTPAKLVNSRPLEAAIREFFSRSQLSQFKDETNPLSSLRHK
RRISALGPGGLTRERAGFDVRDVHRTHYGRICPVETPEGANIGLITSLAA
YARVDELGFIRTPYRRVVGGVVTDEVVYMTATEEDRYTIAQANTPLEGNR
IAAERVVARRKGEPVIVSPEEVEFMDVSPKQVFSVNTNLIPFLEHDDANR
ALMGSNMQTQAVPLIRAQAPVVMTGLEERVVRDSLAALYAEEDGEVAKVD
GNRIVVRYEDGRLVEYPLRRFYRSNQGTALDQRPRVVVGQRVRKGDLLAD
GPASENGFLALGQNVLVAIMPFDGYNFEDAIVISEELLKRDFYTSIHIER
YEIEARDTKLGPERITRDIPHLSEAALRDLDEEGVVRIGAEVKPGDILVG
RTSFKGESEPTPEERLLRSIFGEKARDVKDTSLRVPPGEGGIVVRTVRLR
RGDPGVELKPGVREVVRVYVAQKRKLQVGDKLANRHGNKGVVAKILPVED
MPHLPDGTPVDVILNPLGVPSRMNLGQILETHLGLAGYFLGQRYISPIFD
GAKEPEIKELLAQAFEVYFGKRKGEGFGVDKREVEVLRRAEKLGLVTPGK
TPEEQLKELFLQGKVVLYDGRTGEPIEGPIVVGQMFIMKLYHMVEDKMHA
RSTGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAAHTLQEMLTLKS
DDIEGRNAAYEAIIKGEDVPEPSVPESFRVLVKELQALALDVQTLDEKDN
PVDIFEGLASK

3D structure

• PDB: 7mlj Structural and mechanistic basis of reiterative transcription initiation.
• Chain: C
• Resolution: 3.75 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	C	F394 R1031 M1035	F387 R1024 M1028
BS02	rna	C	R420 N448 K846	R413 N441 K839
BS03	dna	C	R142 P170 R243 R266 L418 R422 V427	R135 P163 R236 R259 L411 R415 V420

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0032549 ribonucleoside binding
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex

External links

• PDB: RCSB:7mlj, PDBe:7mlj, PDBj:7mlj
• PDBsum: 7mlj
• PubMed: 35082149
• UniProt: Q8RQE9|RPOB_THET8 DNA-directed RNA polymerase subunit beta (Gene Name=rpoB)