Structure of PDB 7mib Chain C

Receptor sequence

>7mibC (length=554) Species: 243231 (Geobacter sulfurreducens PCA)

DGSIPLIPVRMLNEHVYCPRLAYLMWVQGEFSHNEFTVDGVIRHRRVDAG
GGVLPSETQEDSRIHARSVSLSSERLGITAKIDLVEGEGAYVSPVDYKRG
KRPHVAGGAYEPERVQLCAQGLLLREHGFASDGGALYFVASRERVPVAFD
DELIGRTLAAIDEMGRTALSGTMPPPLEDSPKCPRCSLVGICLPDEVRFL
SHLSVEPRPIIPADGRGLPLYVQSPKAYVRKDGDCLVIEEERVRVAEARL
GETSQVALFGNATLTTAALHECLRREIPVTWLSYGGWFMGHTVSTGHRNV
ETRTYQYQRSFDPETCLNLARRWIVAKIANCRTLLRRNWRGEGDEAKAPP
GLLMSLQDDMRHAMRAPSLEVLLGIEGASAGRYFQHFSRMLRGGDGEGMG
FDFTTRNRRPPKDPVNALLSFAYAMLTREWTVALAAVGLDPYRGFYHQPR
FGRPALALDMMEPFRPLIADSTVLMAINNGEIRTGDFVRSAGGCNLTDSA
RKRFIAGFERRMEQEVTHPIFKYTISYRRLLEVQARLLTRYLSGEIPAYP
NFVT

3D structure

• PDB: 7mib Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas.
• Chain: C
• Resolution: 5.8 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-
  3.1.12.1: 5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	C	R14 A84 K85 D87	R10 A80 K81 D83
BS02	dna	C	K235 D236 G237 T269 A271 P354 L357 M358 Q361 R365	K231 D232 G233 T265 A267 P350 L353 M354 Q357 R361
BS03	dna	C	T337 R340 R341 K351 T558	T333 R336 R337 K347 T554

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0004527 exonuclease activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:7mib, PDBe:7mib, PDBj:7mib
• PDBsum: 7mib
• PubMed: 34588691
• UniProt: Q74H36|CS4F1_GEOSL CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion (Gene Name=cas4-cas1)