Structure of PDB 7m0e Chain C

Receptor sequence

>7m0eC (length=326) Species: 9606 (Homo sapiens)

WVCAQPSSQKATNHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKS
FHKPVTSYQEACSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELF
SNIWGAGTKTAQMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMP
REEATEIEQTVQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGR
SHRGIFSRLLDSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRR
LDIIVVPYSEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVGR
VLPTPTEKDVFRLLGLPYREPAERDW

3D structure

• PDB: 7m0e Analysis of diverse double-strand break synapsis with Pol lambda reveals basis for unique substrate specificity in nonhomologous end-joining.
• Chain: C
• Resolution: 2.25 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	C	W274 N467 R514 R517 K521 L527 E529 H530	W36 N229 R276 R279 K283 L289 E291 H292
BS02	dna	C	W342 G343 A344 G345 K347 T348 D490 Y505	W104 G105 A106 G107 K109 T110 D252 Y267
BS03	dna	C	W274 R275 G278 Y279 P303 G304 G306 M309	W36 R37 G40 Y41 P65 G66 G68 M71
BS04	dna	C	E465 G468 K472	E227 G230 K234
BS05	MG	C	D427 D429	D189 D191
BS06	DUP	C	R386 G416 S417 R420 G426 D427 D429 Y505 G508 N513	R148 G178 S179 R182 G188 D189 D191 Y267 G270 N275

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:7m0e, PDBe:7m0e, PDBj:7m0e
• PDBsum: 7m0e
• PubMed: 35778389
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)