Structure of PDB 7lo5 Chain C

Receptor sequence

>7lo5C (length=1014) Species: 980427 (Deinococcus wulumuqiensis)

MSLQLVKKFQKRLEDIVAYGGTRNESSVRAAFQQLLSDWAEGSGLRLITE
VTQKAVAGNNVRPDGTLKDSLQQSRGYWESKDEADTLDDEIQKKLAKGYP
RDNIIFEDSRLAVLMQNGEEVQRVDMGDAGALAGLLKLFFEFEPPQVLEF
RKAVDHFKDEMPHLLKILREAADAAEQKADYRGERDHFVEIAKEAINPDF
SPRDAREMLIQHILTGDLFTSVFDNAQYHEDNNIAQQLQQLAATFYKGPV
KRDIAERTKRYYGAIQAAAAQIADHHEKQRFLKALYENFYRAYNPAGAER
LGIFYTPGEIVRFMIEATDTLLEKHFQKELADKGVEILDPATGTGTFITE
LIDFLPKAKLEQKYREELHCNELALLPYYIANLNIEATYAQKMGRYEEFR
NIVLVDTLDNTSGLFGSVTAENLERAKRQNARPVRVIIGNPPYRANQANE
NDNNKNREYKEIDRRIKATYVAASTAQKTKLYDMYSRFLRWATDRLKEDG
IVAFVSNSSFIDSRTFDGFRKEVVKDFDHIYILDMKGNANTSGERRKREG
GNVFNDQIKVGVAVYFLVRSADTKIWYHAVPDFWRAREKLEWLKTTKFED
IEFDHIRPDAKHNWLGQVDEENDWNEFLPVADKDTKQAKGLGQERAIFKL
YSLGVVTNRDEWVYSRAEDELADKVRYFIGRYNEIIKLPLGDLMSRNWEG
DIKMTRATIADAQSRKSYSLEKNSIVPSLYRPFDVLKMYFSKNLNEMQYQ
MPSIFPKGVGENVVIALSGSPAAKPFQVLATDILPSLDLLEKTQCLPFYR
YTMNGERLNNITDYALKAFQTHYADTSISREDIFHYVYAVLHHPAYREKY
ALNLRQEFPRIPFYPEFGRWAAWGRELMALHIGFESVAPYPLKRTDEPPK
NDTPEALALAKKARLKVQRDAAKQPTGAVELDGLTTLAGIPAAAWAYKLG
NRSALEWVLERHKETTPKDATIREKFNTYRFADHKERVIDLLARVTTVSV
ETVRIVGEMPAETM

3D structure

• PDB: 7lo5 Coordination of phage genome degradation versus host genome protection by a bifunctional restriction-modification enzyme visualized by CryoEM.
• Chain: C
• Resolution: 2.86 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	C	T52 N60	T52 N60
BS02	dna	C	N24 E25 S26 N59 N60 R62 D64 Y99	N24 E25 S26 N59 N60 R62 D64 Y99
BS03	dna	C	F304 N448 P449 Y451 A484 Q485 K486 K488 N515 S521 R522 T523 F562 Q565 I566 K567 M762 Y764 Q765 P786 K807	F304 N440 P441 Y443 A476 Q477 K478 K480 N507 S513 R514 T515 F554 Q557 I558 K559 M747 Y749 Q750 P771 K792
BS04	dna	C	N454 Q455 N457 E458 K463 K475 Q485 K486 T487 Y490 A547 N548 T549 R554 K651 N673 D675 K718 R721 R746 D803	N446 Q447 N449 E450 K455 K467 Q477 K478 T479 Y482 A539 N540 T541 R546 K636 N658 D660 K703 R706 R731 D788
BS05	SAM	C	Y286 L301 G302 F304 T306 A341 T346 E372 L373 A374 D406 N448 F496	Y286 L301 G302 F304 T306 A341 T346 E372 L373 A374 D406 N440 F488
BS06	CA	C	E25 D64 E79 S80	E25 D64 E79 S80

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0008168 methyltransferase activity
• GO:0008170 N-methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0009307 DNA restriction-modification system
• GO:0032259 methylation

External links

• PDB: RCSB:7lo5, PDBe:7lo5, PDBj:7lo5
• PDBsum: 7lo5
• PubMed: 33826880
• UniProt: A0A345IJ72