Structure of PDB 7kho Chain C

Receptor sequence

>7khoC (length=432) Species: 1042876 (Pseudomonas putida S16) [Search protein sequence]

GFDYDVVVVGGGFAGATAARECGLQGYRTLLLEARSRLGGRTFTSRFAGQ
EIEFGGAWVHWLQPHVWAEMQRYGLGVVEDPLTNLDKTLIMYNDGSVESI
SPDEFGKNIRIAFEKLCHDAWEVFPRPHEPMFTERARELDKSSVLDRIKT
LGLSRLQQAQINSYMALYAGETTDKFGLPGVLKLFACGGWNYDAFMDTET
HYRIQGGTIGLINAMLTDSGAEVRMSVPVTAVEQVNGGVKIKTDDDEIIT
AGVVVMTVPLNTYKHIGFTPALSKGKQRFIKEGQLSKGAKLYVHVKQNLG
RVFAFADEQQPLNWVQTHDYSDELGTILSITIARKETIDVNDRDAVTREV
QKMFPGVEVLGTAAYDWTADPFSLGAWAAYGVGQLSRLKDLQAAEGRILF
AGAETSNGWHAVIDGAVESGLRAGREVKQLLS

3D structure

PDB

7kho Fast Kinetics Reveals Rate-Limiting Oxidation and the Role of the Aromatic Cage in the Mechanism of the Nicotine-Degrading Enzyme NicA2.

Chain

Resolution

2.69 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

1.4.2.2: nicotine dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NCT	C	L217 Y218 W364 T381 W427	L167 Y168 W314 T331 W377
BS02	FAD	C	G60 G62 A64 E83 A84 R85 G90 R91 T92 G105 G106 W108 P278 V279 V308 P309 I316 F422 A426 W427 A453 V462 I463 A466	G10 G12 A14 E33 A34 R35 G40 R41 T42 G55 G56 W58 P228 V229 V258 P259 I266 F372 A376 W377 A403 V412 I413 A416

Gene Ontology

	Molecular Function
GO:0016491	oxidoreductase activity

View graph for
Molecular Function

External links

PDB	RCSB:7kho, PDBe:7kho, PDBj:7kho
PDBsum	7kho
PubMed	33464876
UniProt	F8G0P2\|NICA2_PSEP6 Nicotine dehydrogenase (Gene Name=nicA2)

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