Structure of PDB 7jz0 Chain C

Receptor sequence

>7jz0C (length=298) Species: 2697049 (Severe acute respiratory syndrome coronavirus 2)

SSQAWQPGVAMPNLYKMQRMLLEKCDLQNYGDSATLPKGIMMNVAKYTQL
CQYLNTLTLAVPYNMRVIHFGAGSDKGVAPGTAVLRQWLPTGTLLVDSDL
NDFVSDADSTLIGDCATVHTANKWDLIISDMYDPKTKNVTKENDSKEGFF
TYICGFIQQKLALGGSVAIKITEHSWNADLYKLMGHFAWWTAFVTNVNAS
SSEAFLIGCNYLGKPREQIDGYVMHANYIFWRNTNPIQLSSYSLFDMSKF
PLKLRGTAVMSLKEGQINDMILSLLSKGRLIIRENNRVVISSDVLVNN

3D structure

• PDB: 7jz0 Structure of SARS-CoV-2 2′- O -methyltransferase heterodimer with RNA Cap analog and sulfates bound reveals new strategies for structure-based inhibitor design
• Chain: C
• Resolution: 2.15 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  2.7.7.50: mRNA guanylyltransferase.
  3.1.13.-
  3.4.19.12: ubiquitinyl hydrolase 1.
  3.4.22.-
  3.4.22.69: SARS coronavirus main proteinase.
  3.6.4.12: DNA helicase.
  3.6.4.13: RNA helicase.
  4.6.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	C	C6823 D6824 L6825 Y6828 S6831 L6834 M6840 N6841 K6844 D6873 K6874 Y6930 K6935 K6968 T6970 E6971 H6972 S6999 S7000	C25 D26 L27 Y30 S33 L36 M42 N43 K46 D75 K76 Y132 K137 K170 T172 E173 H174 S201 S202
BS02	SAH	C	N6841 Y6845 G6869 G6871 P6878 G6879 D6897 L6898 D6912 C6913 D6928 M6929 Y6930	N43 Y47 G71 G73 P80 G81 D99 L100 D114 C115 D130 M131 Y132

Gene Ontology

Molecular Function

• GO:0004483 mRNA (nucleoside-2'-O-)-methyltransferase activity

External links

• PDB: RCSB:7jz0, PDBe:7jz0, PDBj:7jz0
• PDBsum: 7jz0
• UniProt: P0DTD1|R1AB_SARS2 Replicase polyprotein 1ab (Gene Name=rep)