Structure of PDB 7frw Chain C

Receptor sequence

>7frwC (length=425) Species: 9606 (Homo sapiens)

ELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASR
SVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYR
PVAIALDTKGPGPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRA
ALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKV
FLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGA
DCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSR
DPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRS
AQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVG
DLVIVVTGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 7frw Tuning liver pyruvate kinase activity up or down with a new class of allosteric modulators.
• Chain: C
• Resolution: 1.737 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	OA0	C	F38 L39	F20 L21
BS02	OA0	C	F38 L365 Q405 L406 E409	F20 L247 Q287 L288 E291
BS03	FBP	C	L443 T444 T445 T446 S449 W494 R501 G526 R528 P529 G530 S531 Y533 T534	L325 T326 T327 T328 S331 W376 R383 G408 R410 P411 G412 S413 Y415 T416
BS04	OXL	C	K282 E284 A305 G307 D308 T340	K164 E166 A187 G189 D190 T222
BS05	MG	C	E284 D308	E166 D190

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:7frw, PDBe:7frw, PDBj:7frw
• PDBsum: 7frw
• PubMed: 36753880
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)