Structure of PDB 7ddh Chain C

Receptor sequence

>7ddhC (length=996) Species: 9823 (Sus scrofa)

KERDMDELKKEVSMDDHKLSLDELHRKYGTDLSRGLTPARAAEILARDGP
NALTPPPTTPEWVKFCRQLFGGFSMLLWIGAILCFLAYGIQAATEEEPQN
DNLYLGVVLSAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRNGEK
MSINAEEVVVGDLVEVKGGDRIPADLRIISANGCKVDNSSLTGESEPQTR
SPDFTNENPLETRNIAFFSTNCVEGTARGIVVYTGDRTVMGRIATLASGL
EGGQTPIAAEIEHFIHIITGVAVFLGVSFFILSLILEYTWLEAVIFLIGI
IVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDK
TGTLTQNRMTVAHMWSDNQIHEADTTENQSGVSFDKTSATWLALSRIAGL
CNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYTKI
VEIPFNSTNKYQLSIHKNPNTAEPRHLLVMKGAPERILDRCSSILIHGKE
QPLDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVN
FPLDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAI
AKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQ
LDDILKYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPASKK
ADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIA
YTLTSNIPEITPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQA
ESDIMKRQPRNPKTDKLVNEQLISMAYGQIGMIQALGGFFTYFVILAENG
FLPIHLLGLRVNWDDRWINDVEDSYGQQWTYEQRKIVEFTCHTPFFVTIV
VVQWADLVICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMGVA
LRMYPLKPTWWFCAFPYSLLIFVYDEVRKLIIRRRPGGWVEKETYY

3D structure

• PDB: 7ddh Binding of cardiotonic steroids to Na + ,K + -ATPase in the E2P state.
• Chain: C
• Resolution: 3.46 Å

Enzymatic activity

• Enzyme Commision number: 7.2.2.13: Na(+)/K(+)-exchanging ATPase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CLR	C	E868 T979 C983	E848 T959 C963
BS02	MG	C	X369 T371 D710	X349 T351 D690
BS03	NA	C	G272 K719 D740	G252 K699 D720
BS04	MG	C	E327 E779 D804	E307 E759 D784
BS05	DGX	C	Q111 Q119 N122 L125 E312 G319 V322 A323 F783 T797	Q91 Q99 N102 L105 E292 G299 V302 A303 F763 T777
BS06	CLR	C	W924 P978 W981 F982 F985	W904 P958 W961 F962 F965

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0005215 transporter activity
• GO:0005391 P-type sodium:potassium-exchanging transporter activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008556 P-type potassium transmembrane transporter activity
• GO:0016887 ATP hydrolysis activity
• GO:0030955 potassium ion binding
• GO:0031402 sodium ion binding
• GO:0046872 metal ion binding
• GO:0051117 ATPase binding

Biological Process

• GO:0002028 regulation of sodium ion transport
• GO:0006813 potassium ion transport
• GO:0006814 sodium ion transport
• GO:0006883 intracellular sodium ion homeostasis
• GO:0010248 establishment or maintenance of transmembrane electrochemical gradient
• GO:0030007 intracellular potassium ion homeostasis
• GO:0036376 sodium ion export across plasma membrane
• GO:0055085 transmembrane transport
• GO:0086009 membrane repolarization
• GO:1902600 proton transmembrane transport
• GO:1990573 potassium ion import across plasma membrane

Cellular Component

• GO:0005886 plasma membrane
• GO:0005890 sodium:potassium-exchanging ATPase complex
• GO:0016020 membrane
• GO:0016323 basolateral plasma membrane
• GO:0030424 axon
• GO:0042383 sarcolemma
• GO:0042470 melanosome
• GO:0042995 cell projection

External links

• PDB: RCSB:7ddh, PDBe:7ddh, PDBj:7ddh
• PDBsum: 7ddh
• PubMed: 33318128
• UniProt: P05024|AT1A1_PIG Sodium/potassium-transporting ATPase subunit alpha-1 (Gene Name=ATP1A1)