Structure of PDB 7a24 Chain C

Receptor sequence

>7a24C (length=693) Species: 3712 (Brassica oleracea)

PTQILPPRNPVGGARVHFSNPEDAIEVFVDGYAVKVPKGFTVLQACEVAG
VDIPRFCYHSRLSIAGNCRMCLVEVEKSPKPVASCAMPALPGMKIKTDTP
IAKKAREGVMEFLLMNHPLDCPICDQGGECDLQDQSMAFGSDRGRFTEMK
RSVVDKNLGPLVKTVMTRCIQCTRCVRFASEVAGVQDLGILGRGSGEEIG
TYVEKLMTSELSGNVIDICPVGALTSKPFAFKARNWELKATETIDVSDAV
GSNIRVDSRGPEVMRIIPRLNEDINEEWISDKTRFCYDGLKRQRLSDPMI
RDSDGRFKAVSWRDALAVVGDIIHQVKPDEIVGVAGQLSDAESMMVLKDF
VNRMGSDNVWCEGTAAGVDADLRYSYLMNTSISGLENADLFLLIGTQPRV
EAAMVNARICKTVRASNAKVGYVGPPAEFNYDCKHLGTGPDTLKEIAEGR
HPFCTALKNAKNPAIIVGAGLFNRTDKNAILSSVESIAQANNVVRPDWNG
LNFLLQYAAQAAALDLGLIQQSAKALESAKFVYLMGADDVNVDKIPKDAF
VVYQGHHGDKAVYRANVILPASAFTEKEGTYENTEGFTQQTVPAVPTVGD
ARDDWKIVRALSEVSGVKLPYNSIEGVRSRIKSVAPNLVHTDEREPAAFG
PSLKPECKEAMSTTPFQTVVENFYMTNSITRASKIMAQCSAVL

3D structure

• PDB: 7a24 Specific features and assembly of the plant mitochondrial complex I revealed by cryo-EM.
• Chain: C
• Resolution: 3.8 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.2: NADH:ubiquinone reductase (H(+)-translocating).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	C	H166 D169 C170 C173 G176 C179 L181 Q182 V270 G271	H117 D120 C121 C124 G127 C130 L132 Q133 V221 G222
BS02	SF4	C	C218 I219 Q220 C221 T222 R223 C224 I248 C268 P269	C169 I170 Q171 C172 T173 R174 C175 I199 C219 P220
BS03	FES	C	R104 C106 Y107 C117 C120 C134	R55 C57 Y58 C68 C71 C85

Gene Ontology

Molecular Function

• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0016491 oxidoreductase activity
• GO:0016651 oxidoreductase activity, acting on NAD(P)H
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051537 2 iron, 2 sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0009853 photorespiration
• GO:0042773 ATP synthesis coupled electron transport
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0009507 chloroplast
• GO:0009536 plastid
• GO:0016020 membrane
• GO:0031966 mitochondrial membrane
• GO:0045271 respiratory chain complex I

External links

• PDB: RCSB:7a24, PDBe:7a24, PDBj:7a24
• PDBsum: 7a24
• PubMed: 33060577
• UniProt: Q9FGI6|NDUS1_ARATH NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial (Gene Name=EMB1467)