Structure of PDB 6zyw Chain C

Receptor sequence

>6zywC (length=4433) Species: 312017 (Tetrahymena thermophila SB210)

SKRIAWMKTTICDSLQLKDMIVEESFQYEKNKNLLEQFLSGEGLNKIFAY
YQVQEQAQNDDIKDTGAQDPVLFFTTGDLEKIQDKAVWFLRITNPADDKK
KASQQDGNDNDIIFGEITPNTVPMLNALMESVYSRQIDHIITEKIQFWGV
AEEEQVLEFQQHSNKFSSEVREAINLMSPGTEHFKLDYEAISGLSESEKM
QHYEMKFNEWINLISSQLNRKDEKDAGPATELIYWRSRMQKITNWSEQLK
SKDFQIVKASLQRHKNHDNQRPRGDESLNEAKDNVKYLTTLEKFIEPLYN
GTPQQIIDTLPALMNAIKMIHTIARFYNTTDKMTGLFIKITNQMIKNCKD
RILNMIWEQDPAELIEVLGSCIKLYCEYKKCYNDTKEKVADMPKGKTFDF
SDAQIFGKFDTFVRRLQKLIEIFSNIQQFNALAKHNLEGMDVLTNKFKKI
IDDFKKKGHNLLDTANNKFDRDWVEFNVEISHLDGELQNFIDNNFNRFRN
IEYSLKLLHKFQSTIKRDSLKHNLTSRYNAILHNYATELDTIQRVFQDQK
SNPPLVRNMPPEAGKIIWARHLFQKITGPINIFPENVINSTEIRRYYGSY
NTLGKQLTIYEMWFYQDWVNKIEQSKAALQATLIVRHDENKKLYVNFDLE
IMQLIREAKCLDRQGIEIPESARIILLQEDKFKTYYNELLYALKEYERIN
SKIKPICKNLLLPHIEDLDLKLRPGMVTLTWTSMNIESYLYYVHQGLKKL
EQLIINVNDIIENRIENNLKTVSKVVLVHLPNVEVERAVDDLLQTIMLYP
LDPHVDPVLPEETKRIKRYYFWYFYQALLNSTQNSLNAMKYRVCGKKIPG
ANTLQNLKPFFQVEVQLNGDKVTLNPSLQEIQKSINRAATAVLRCSKHLY
NWDQQNKDSTDKATFYDMIACDKEIVKVILLLTGSIQGTKNKVNEFLSGF
TKFEWLCKESIQESIKKFSKNGPTLQNYEDQLKKFSQIEEEIEKIVPTYK
IGAMELMTHNICTSLSTWAKEWKLQYSQDLHKRARQLLDSLTEQTKMLST
KLSKPVKDIDSLGYVMETLEQIRKEQAEIDMKFNPVQEMYSLLDNYLPGG
ITDKDEMDARSLLRRNWDILIQQAEIKGKEYQHKQAIYLKELKQSIKDFT
NQVSIFRRDYEKNGPMVEGISPAEAMERLRRFEDEYDVKYQMYKINARGE
NLFGLQNQKYPELEKTDAEIKNLNKLYNLYDSVIKNIQQFKEKSWQDVSK
DDLAKMEEDAGKYGEQCSRLPKDLKEWQAYRDLKNYIDSLREQLPLIISL
KKPSIMPRHWEKIKEITNTKLNYENPDQFYIEEIMGAKLLDFREDIEDIT
ESADKQLKIRTGLDEINLYWNDMQFQFGIWGKRDVPCMLNGLIVGTILER
LEEDQLQLSTFNSQRHVTPFKAEVENLIRTFSDVNDTLDMWVKVQKLWTS
LEPVFTGGDIARQMPLQAKQFQGIDKNWMKIMEKAVETKKVIPCCQNDML
KDFLPDLNRKLEDCQKMLEAYLEGKRKKFPRFYFVSNPTLLKILSQGSEP
TSIQEDFEKLFDAITKVTFESAKDKKNPALKQITQIQQVIGRNEENISLT
GYYVKCEGNIEDWLKKLEQNMQQTLKDIASAAAQQVFQVGLKEFVSSQAS
QIALLGLQILWTSKVNEGLERLSRNERNAMDIKRNEIKEHMNILSSMCLE
DLNGAVERTKVETLVTIQVHQKDISMDLKCKDVNDFEWQKQTRIAWKTDI
DECIISITDWDSPYSYEFLGAKERLCITPLTDRCYITLAQAMSMYYGGAP
AGPAGTGKTETVKDLGRTLGVFVVVTNCSDQHRYRDMAKIFKGLVQSGLW
GCFDEFNRIDLEVLSVVAMQVESITTARKQHMKKFMFPEEEIEIELIPTV
SYFITMNPGYAGRQELPENLKVLFRGVSMMVPDREIIIKVKLASVGYLQI
DLLAKKFNVLYRLCEEQLSKQRHYDFGLRNILSVLRTAGNTKRQEIKSDE
EMLLMRSLRDMNLSKLVADDIPLFNGLLADIFPKLKEVPKKLYPDVEKKI
PEEINAESYLINTPSFQLKIIQLYETCLVRHGFMLVGPTGSGKSTIMKIL
TEVLTKLGSPHKIVIMNPKAITAEQMYGVKSEISDDWIPGVFSTIWAKSN
NRALKHTTWITCDGPVDAIWIENLNTVLDDNKILTLANGERIAMTENCKV
VFEVENLNNASPATVSRCGQVYVSPTDLGYEAVIEGWIRNRKASGRAEES
DKLGNILRKYLINMRFIELQSKECKEPMMDTSPVISVINILNLLTGCLQY
FVQTQRTLSEQEYEKFIVYSMAWAIGGIYEAQDRVRFHELLLAKNAPIPQ
KGKENETVFDYYVSQDYLDWKICSPEEWVPPQSLQFSQLLLPTLDSFRAE
MLLNFILTQPKSHTCSNSALLIGGSGTAKTSSVLLYCNKFDPQKMLFKRT
NFSSATSPFMFQSTIEAECDFKVGKEFAPPGNKMMTIFIDDMSMPFVNKW
GDQITLELVRQLIETGGFYMLDKTQRGNQRKMKNLQYIGAMNHPGGGRND
IPNRLKRQFFIFNMILPLSIEGIYGPIIKHMFKQKYFSDSTYKVIESLTS
ATIALWNKVKSTMLPTPAKFHYVFNMRELSRIFKGILTCKKDTINDAPKS
MKIKPELFLVGLWRHEAERVLADKLVNNKDKDTVMGYIQEVSLESFSQIE
NEILEKYSSEKTFLFCDFLRPDVINEDGIIEEEAPKIYEAIDSLTELRKR
CNFLLSFYNDRNPSKKMPLVLFDDALKHLLRISRIIRQPRSSGLLVGVGG
SGKQSLTRLAGFIGKNLIQQIIVTKTYSDKDLKEDIKKGFDDAGHLGKQV
TFLMTDSEVKKEEFLEYINMVLSTGEIPNLLAKDEREVWLGDISQAYCKE
KNLGNIDPPQSELWTYFVDRVRDNFHIMLCFSPVGQKFRERARKFPALFN
ECTIDWFLPWPEEALVSVAETFIKNFDKLDTKEETKQELMKHMGNVHLMV
NEICDEYYQKMRRQVYVTPKSFLSYLNSYKTLYIEKYDELDQQEESFKIG
LNKIQEATITINQMEISLKEEEIQLNEATEKTNQLLANLDKESKKANQKG
EEVAATNKQCEIQAEQISKEKEEAERELEAALPALRRAQEAVDSIESKDI
VELKANKKPLDIIKYIMDAVLVFFKARLSYDESGIQTLGDMNFMKKLKEF
EKDSINEETIELLEPYLNQSEFNDTFATKASKAAAGILKWAFAIYEYHQK
SKIVKPKRIQVAIAEGRQAIALKELEKAREDLAQIQAYIKNLKDVYTKQM
EEKNELEMKAAKTKKKINTARTLITSLSGEKDRWGKGAQDISDQKRKLVG
NVSLSTAFISYCGPFNAEYRNKLAQQRFVVDMKKRGVPVTPGLELTSFLI
DDATIGEWNLQGLPKDDLSIQNGIMVTNSARYPLFIDPQGQGQNWIRNKL
SAITTLSHPKFKDMFLKYCMESGLTLIVENIENEVDPMMDPVLELSKEFK
LFMTCRLANPSFSPELSAKTTIIDFTVTQSGLEQQLLGKVISKEQKALED
SLNQLLADVNQNQKDLQRLDKNLLERLINSQGNLLDDTELMDVLNNTKTQ
AKEVAAKLIDAEIKTKEINEKREQYRPVAIRGSAIYFTMIEVSLVNWMYN
SSLEQFLKLFIESIDLSEKAQLPSNRVKNIISFLTFHVYRYVNRGLFEKD
KITFILMMAFKILTTAGTISSGDVSLFLKSQKQISYLEDNQWLNILALSK
PFFKELPDLISRSENQWRNWIDKNDPENFPIPDFAESINQEKEIGSFISL
CLVRSLRNDRTLIATQNFISNVLGKEFTDPISYPIEGIWQESSNMDPVLF
LLSAGADPTSSIDELAKKKKKFPCEKVSMGEGQERVARQVIMKGFVEGGW
VILQNCHLGLKFMEEIETLVSPINQIHEDFRLWITCEQHPKFPLGLLQKT
LKVTNEPPKGLKAGLYKTFTTIITQEFIDKVDHSNWRSLIFTICFLHSIV
IERKKFGPLGWCVPYEYNYSDLEASLLYIEKYLTNLMSTPQPNSHNLPIS
MNVVRYMICEVQYGGRITDDLDRELFITYGETYLKDGIFGNDYFFPQNPS
AELIKYQEYIAKVPTVDNPEVFGLHSNADLTFRLKESKEMINTVMETRPR
EEIVQDKAKDMLKNLPPDYNDVEVRELVSKLGGPNPKTSTERGMTVPLNI
FLYQEVTRMQRVIGLVRKTLQDTILAIDGQIIMTPEILEAINAIYDAKVP
NSWLYDPSGAEISWLLPNLGSWSTSLSDRNKQLNDWLRSGQRPILFWLTG
FFNPQGFLTGMKQEVTRNHKKEAWSLDDVVYSTTVKEREKEPAEGVYIKG
LYLEGCKWSKNGLDDSDPKKIFADLPILHVSAINKSNTYLCPVYKYPKRT
DKYLIFRVGLPCEGSNNPSHWKLRGVALLCSTE

3D structure

• PDB: 6zyw Shulin packages axonemal outer dynein arms for ciliary targeting.
• Chain: C
• Resolution: 8.78 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	C	A1874 G1875 G1877 K1878	A1804 G1805 G1807 K1808
BS02	ATP	C	L2130 I2131 G2162 K2163 S2164	L2060 I2061 G2092 K2093 S2094
BS03	ADP	C	L2461 G2496 T2497 A2498 K2499	L2391 G2426 T2427 A2428 K2429
BS04	ADP	C	G2869 G2870 S2871 G2872 Q2874 S2875	G2799 G2800 S2801 G2802 Q2804 S2805

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008569 minus-end-directed microtubule motor activity
• GO:0016887 ATP hydrolysis activity
• GO:0045505 dynein intermediate chain binding
• GO:0051959 dynein light intermediate chain binding

Biological Process

• GO:0007018 microtubule-based movement

Cellular Component

• GO:0005737 cytoplasm
• GO:0005856 cytoskeleton
• GO:0005858 axonemal dynein complex
• GO:0005874 microtubule
• GO:0005929 cilium
• GO:0030286 dynein complex

External links

• PDB: RCSB:6zyw, PDBe:6zyw, PDBj:6zyw
• PDBsum: 6zyw
• PubMed: 33632841
• UniProt: Q22A67