Structure of PDB 6zhu Chain C

Receptor sequence

>6zhuC (length=995) Species: 4932 (Saccharomyces cerevisiae)

AAGEIDESLYSRQLYVLGKEAMLKMQTSNVLILGLKGLGVEIAKNVVLAG
VKSMTVFDPEPVQLADLSTQFFLTEKDIGQKRGDVTRAKLAELNAYVPVN
VLDSLDDVTQLSQFQVVVATDTVSLEDKVKINEFCHSSGIRFISSETRGL
FGNTFVDLGDEFTVLDPTGEEPRTGMVSDIEPDGTVTMLDDNRHGLEDGN
FVRFSEVEGLDKLNDGTLFKVEVLGPFAFRIGSVKEYGEYKKGGIFTEVK
VPRKISFKSLKQQLSNPEFVFSDFAKFDRAAQLHLGFQALHQFAVRHNGE
LPRTMNDEDANELIKLVTDLSVQQPEVLGEGVDVNEDLIKELSYQARGDI
PGVVAFFGGLVAQEVLKACSGKFTPLKQFMYFDSLESLPDPKNFPRNEKT
TQPVNSRYDNQIAVFGLDFQKKIANSKVFLVGSGAIGCEMLKNWALLGLG
SGSDGYIVVTDNDSIEKSNLNRQFLFRPKDVGKNKSEVAAEAVCAMNPDL
KGKINAKIDKVGPETEEIFNDSFWESLDFVTNALDNVDARTYVDRRCVFY
RKPLLESGTLGTKGNTQVIIPRLTESYSSSRDPPEKSIPLCTLRSFPNKI
DHTIAWAKSLFQGYFTDSAENVNMYLTQPNFVEQTLKQSGDVKGVLESIS
DSLSSKPHNFEDCIKWARLEFEKKFNHDIKQLLFNFPKDAKTSNGEPFWS
GAKRAPTPLEFDIYNNDHFHFVVAGASLRAYNYGIKSDDSNSKPNVDEYK
SVIDHMIIPEFTPNGSDEIDQLVSSLPDPSTLAGFKLEPVDFEKDDDTNH
HIEFITACSNCRAQNYFIETADRQKTKFIAGRIIPAIATTTSLVTGLVNL
ELYKLIDNKTDIEQYKNGFVNLALPFFGFSEPIASPKGEYNNKKYDKIWD
RFDIKGDIKLSDLIEHFEKDEGLEITMLSYGVSLLYASFFPPKKLKERLN
LPITQLVKLVTKKDIPAHVSTMILEICADDKEGEDVEVPFITIHL

3D structure

• PDB: 6zhu ATP induced conformational changes facilitate E1-E2 disulfide bridging in the ubiquitin system
• Chain: C
• Resolution: 3.18 Å

Enzymatic activity

• Enzyme Commision number: 6.2.1.45: E1 ubiquitin-activating enzyme.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	C	R21 D470 R481 K494 K519 V520 A542 L543 D544 N545	R12 D461 R472 K485 K510 V511 A533 L534 D535 N536

Gene Ontology

Molecular Function

• GO:0004839 ubiquitin activating enzyme activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008641 ubiquitin-like modifier activating enzyme activity
• GO:0016874 ligase activity

Biological Process

• GO:0006511 ubiquitin-dependent protein catabolic process
• GO:0006974 DNA damage response
• GO:0016567 protein ubiquitination
• GO:0036211 protein modification process
• GO:0043412 macromolecule modification

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm

External links

• PDB: RCSB:6zhu, PDBe:6zhu, PDBj:6zhu
• PDBsum: 6zhu
• UniProt: P22515|UBA1_YEAST Ubiquitin-activating enzyme E1 1 (Gene Name=UBA1)