Structure of PDB 6vcj Chain C

Receptor sequence
>6vcjC (length=185) Species: 9606 (Homo sapiens) [Search protein sequence]
GSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVI
MGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTE
QPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEI
DLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND
3D structure
PDB6vcj The Structural Basis for Nonsteroidal Anti-Inflammatory Drug Inhibition of Human Dihydrofolate Reductase.
ChainC
Resolution2.34 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 1.5.1.3: dihydrofolate reductase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 LG3 C V8 E30 F34 V7 E29 F33 BindingDB: Ki=180000nM
BS02 NPX C F34 S59 I60 N64 L67 R70 F33 S58 I59 N63 L66 R69 MOAD: Ki=0.11mM
BindingDB: Ki=3400000nM
BS03 NAP C V8 A9 I16 G20 D21 G53 K54 K55 T56 L75 S76 R77 E78 R91 V115 G117 T146 V7 A8 I15 G19 D20 G52 K53 K54 T55 L74 S75 R76 E77 R90 V114 G116 T145
Gene Ontology
Molecular Function
GO:0000900 mRNA regulatory element binding translation repressor activity
GO:0003723 RNA binding
GO:0003729 mRNA binding
GO:0004146 dihydrofolate reductase activity
GO:0005542 folic acid binding
GO:0016491 oxidoreductase activity
GO:0050661 NADP binding
GO:0070402 NADPH binding
GO:1990825 sequence-specific mRNA binding
Biological Process
GO:0006729 tetrahydrobiopterin biosynthetic process
GO:0006730 one-carbon metabolic process
GO:0017148 negative regulation of translation
GO:0031103 axon regeneration
GO:0031427 response to methotrexate
GO:0046452 dihydrofolate metabolic process
GO:0046653 tetrahydrofolate metabolic process
GO:0046654 tetrahydrofolate biosynthetic process
GO:0046655 folic acid metabolic process
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:2000121 regulation of removal of superoxide radicals
Cellular Component
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:6vcj, PDBe:6vcj, PDBj:6vcj
PDBsum6vcj
PubMed32658475
UniProtP00374|DYR_HUMAN Dihydrofolate reductase (Gene Name=DHFR)

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