Structure of PDB 6uqo Chain C

Receptor sequence

>6uqoC (length=578) Species: 83333 (Escherichia coli K-12)

MENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFK
ALLKQLEQDTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRV
IGSTTYQEFSNIFEKDRALARRFQKIDITEPSIEETVQIINGLKPKYEAH
HDVRYTAKAVRAAVELAVKYINDRHLPDKAIDVIDEAGARARLMPVSKRK
KTVNVADIESVVARIARIPEKSVSQSDRDTLKNLGDRLKMLVFGQDKAIE
ALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQLSKALGIELLR
FDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEIEK
AHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIG
LIHQDNSTDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVE
LQVQLDQKGVSLEVSQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLAN
ELLFGSLVDGGQVTVALDKEKNELTYGF

3D structure

• PDB: 6uqo Conformational plasticity of the ClpAP AAA+ protease couples protein unfolding and proteolysis.
• Chain: C
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 3.4.21.92: endopeptidase Clp.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	C	K258 Y259 R260	K90 Y91 R92
BS02	peptide	C	G539 Y540 V541	G371 Y372 V373
BS03	AGS	C	L188 I189 S216 G217 V218 G219 K220 T221 I357	L20 I21 S48 G49 V50 G51 K52 T53 I189
BS04	AGS	C	V460 F461 T497 G498 V499 G500 K501 T502 E503 N606 A701 R702	V292 F293 T329 G330 V331 G332 K333 T334 E335 N438 A533 R534
BS05	AGS	C	R339 R340	R171 R172

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity

External links

• PDB: RCSB:6uqo, PDBe:6uqo, PDBj:6uqo
• PDBsum: 6uqo
• PubMed: 32313240
• UniProt: P0ABH9|CLPA_ECOLI ATP-dependent Clp protease ATP-binding subunit ClpA (Gene Name=clpA)