Structure of PDB 6uk3 Chain C

Receptor sequence

>6uk3C (length=475) Species: 1257118 (Acanthamoeba castellanii str. Neff)

TQTQTQPAGNAFQYEIRDISLAEFGRKEINLAEHEMPGLMQTREKYGAEQ
PLKGVRLAGSLHMTIQTAVLIETLQALGANVRWCSCNIFSTQDHAAAAIV
AAGTPVFAWKGETLEEYWECTWKTLLFPDDMGPQLIVDDGGDATLMVHRG
FYAEDNPSILDDDEGSEELAIVNKLLKRIQKEKPGYWHKIVPELKGVSEE
TTTGVHRLYEMMKEGKLLFPALNVNDSVTKSKFDNVYGCRHSLVDAIMRA
TDVMLSGKVACVLGYGDVGKGSAESLKGQGARVVVTEVDPICALQACMAG
YEVVRIEDVLDKAEIFVTTTGNCDIIRIEHMEKMRHNAIVCNIGHFDNEI
QVKALKEFPGIKRIEIKPQVDQFVFPDGHAIVLLAEGRLVNLGCATGHPS
FVMSNSFTNQTLAQISLWKEKYELGVYTLPKKLDEEVARLHLEKLGAKLT
VLTDKQAKYLGIAKDGPYKPDHYRY

3D structure

• PDB: 6uk3 Crystal Structure of S-adenosyl-L-homocysteine hydrolase from Acanthamoeba castellanii with bound NAD and Adenosine
• Chain: C
• Resolution: 1.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H64 S87 S92 D141 E202 N227 K232 D236 N237 C241 H347 H400 S408 Q412
• Catalytic site (residue number reindexed from 1): H62 S85 S90 D139 E200 N225 K230 D234 N235 C239 H345 H398 S406 Q410
• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	C	K471 Y475	K469 Y473
BS02	NAD	C	T203 T204 T205 N237 G268 D269 V270 E289 V290 T322 N324 I327 I345 G346 N393 H400	T201 T202 T203 N235 G266 D267 V268 E287 V288 T320 N322 I325 I343 G344 N391 H398
BS03	ADN	C	H64 T66 Q68 D141 E202 T203 K232 D236 L391 L394 H400 M405	H62 T64 Q66 D139 E200 T201 K230 D234 L389 L392 H398 M403

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:6uk3, PDBe:6uk3, PDBj:6uk3
• PDBsum: 6uk3
• UniProt: L8H6B5