Structure of PDB 6tyf Chain C

Receptor sequence

>6tyfC (length=1114) Species: 1773 (Mycobacterium tuberculosis)

SNNSVPGAPNRVSFAKLREPLEVPGLLDVQTDSFEWLIGSPRWRESAAER
GDVNPVGGLEEVLYELSPIEDFSGSMSLSFSDPRFDDVKAPVDECKDKDM
TYAAPLFVTAEFINNNTGEIKSQTVFMGDFPMMTEKGTFIINGTERVVVS
QLVRSPGVYFDETIDKSTDKTLHSVKVIPSRGAWLEFDVDKRDTVGVRID
RKRRQPVTVLLKALGWTSEQIVERFGFSEIMRSTLEKDNTVGTDEALLDI
YRKLRPGEPPTKESAQTLLENLFFKEKRYDLARVGRYKVNKKLGLHVGEP
ITSSTLTEEDVVATIEYLVRLHEGQTTMTVPGGVEVPVETDDIDHFGNRR
LRTVGELIQNQIRVGMSRMERVVRERMTTQDVEAITPQTLINIRPVVAAI
KEFFGTSQLSQFMDQNNPLSGLTHKRRLSALGPGGLSRERAGLEVRDVHP
SHYGRMCPIETPEGPNIGLIGSLSVYARVNPFGFIETPYRKVVDGVVSDE
IVYLTADEEDRHVVAQANSPIDADGRFVEPRVLVRRKAGEVEYVPSSEVD
YMDVSPRQMVSVATAMIPFLEHDDANRALMGANMQRQAVPLVRSEAPLVG
TGMELRAAIDAGDVVVAEESGVIEEVSADYITVMHDNGTRRTYRMRKFAR
SNHGTCANQCPIVDAGDRVEAGQVIADGPCTDDGEMALGKNLLVAIMPWE
GHNYEDAIILSNRLVEEDVLTSIHIEEHEIDARDTKLGAEEITRDIPNIS
DEVLADLDERGIVRIGAEVRDGDILVGKVTPKGETELTPEERLLRAIFEV
RDTSLKVPHGESGKVIGIRVFSREDEDELPAGVNELVRVYVAQKRKISDG
DKLAGRHGNKGVIGKILPVEDMPFLADGTPVDIILNTHGVPRRMNIGQIL
ETHLGWCAHSGWKVDAAKGVPDWAARLPDELLEAQPNAIVSTPVFDGAQE
AELQGLLSCTLPNRDGDVLVDADGKAMLFDGRSGEPFPYPVTVGYMYIMK
LHHLVDDKIHARSTGPYSMITQQPLGGKAQFGGQRFGEMECWAMQAYGAA
YTLQELLTIKSDDTVGRVKVYEAIVKGENIPEPGIPESFKVLLKELQSLC
LNVEVLSSDGAAIE

3D structure

• PDB: 6tyf RNA extension drives a stepwise displacement of an initiation-factor structural module in initial transcription.
• Chain: C
• Resolution: 3.8 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	C	K218 F439 G1059 R1067 M1071	K191 F412 G1027 R1035 M1039
BS02	dna	C	F99 R181 K203 G209 W211 D227 R228 R282 E285 I370 D371 R376 L463 E466 R467 V472	F72 R154 K176 G182 W184 D200 R201 R255 E258 I343 D344 R349 L436 E439 R440 V445
BS03	rna	C	Q435 R465 N493 I497 Q614 K884 K892 H1035	Q408 R438 N466 I470 Q587 K852 K860 H1003

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0005515 protein binding
• GO:0016779 nucleotidyltransferase activity
• GO:0032549 ribonucleoside binding
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0046677 response to antibiotic

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005829 cytosol
• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall

External links

• PDB: RCSB:6tyf, PDBe:6tyf, PDBj:6tyf
• PDBsum: 6tyf
• PubMed: 32127479
• UniProt: P9WGY9|RPOB_MYCTU DNA-directed RNA polymerase subunit beta (Gene Name=rpoB)