Structure of PDB 6t66 Chain C

Receptor sequence

>6t66C (length=440) Species: 666 (Vibrio cholerae)

KVPPHSLEAEQSVIGGLLLDNERWDTVSEHVMTQDFYSRPHRLIFDGVKS
ILEAGKPLDLITLSEYLEQREQLEDVGGFAYLADLAKNTPSAANINAYAE
IVAERALVRNLIGVANEIADAGYDPQGRNAEDLLDLAESKVFAIAEARTS
ENEGPKNVDSILERTLERIELLYKTPQDGVTGVNTGFTDLNKKTAGLQGS
DLIIVAARPSMGKTTFAMNLCENAAMEQDKPVLIFSLEMPAEQIMMRMLA
SLSRVDQTKIRTGQLDDEDWARISSTMGILMEKKNMYIDDSSGLTPTEVR
SRARRIAREHGGLSLIMVDYLQLMRVPALTDNRTLEIAEISRSLKALAKE
LNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVY
HPDSPLKGTAEIIIGKQRNGPIGSVRLTFQGHYSRFDNYA

3D structure

• PDB: 6t66 Study of the DnaB:DciA interplay reveals insights into the primary mode of loading of the bacterial replicative helicase.
• Chain: C
• Resolution: 3.9 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.12: DNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GDP	C	Q438 R439 N440 G441	Q417 R418 N419 G420
BS02	ALF	C	K437 R439	K416 R418
BS03	GDP	C	S231 G233 K234 T235 T236 R268 Q278 G452	S210 G212 K213 T214 T215 R247 Q257 G431
BS04	ALF	C	K234 E259 Y341	K213 E238 Y320

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003678 DNA helicase activity
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006260 DNA replication
• GO:0006268 DNA unwinding involved in DNA replication
• GO:0006269 DNA replication, synthesis of primer

Cellular Component

• GO:0005829 cytosol
• GO:1990077 primosome complex

External links

• PDB: RCSB:6t66, PDBe:6t66, PDBj:6t66
• PDBsum: 6t66
• PubMed: 34107018
• UniProt: Q9KUY7