Structure of PDB 6sjf Chain C

Receptor sequence

>6sjfC (length=1121) Species: 562 (Escherichia coli)

MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQWLQMTL
SQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLP
QLLEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWE
TGHLVEGLGEAQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESA
TTCPPGLPSRVFICGISALPPVYLQALQALGKHIEIHLLFTNPCRYYWGD
IKDPAYLAKLLTRQRRHSFEDRELPLFRDSENAGQLFNSDGEQDVGNPLL
ASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNLLHNIQSDILELENRAV
AGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDRLLAMLEED
PTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNES
GIRWGIDDDNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPY
DESSGLIAELVGHLASLLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFL
PDAETEAAMTLIEQQWQAIIAEGLGAQYGDAVPLSLLRDELAQRLDQERI
SQRFLAGPVNICTLMPMRSIPFKVVCLLGMNDGVYPRQLAPLGFDLMSQK
PKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNSERFPSVLVQE
LIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGERQ
SYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQ
MRLQVNFRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRR
FRAAGDLPYGAFGEIFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGV
QITGWLPQVQPDGLLRWRPSLLSVAQGMQLWLEHLVYCASGGNGESRLFL
RKDGEWRFPPLAAEQALHYLSQLIEGYREGMSAPLLVLPESGGAWLKTCY
DAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDIWYQRLWRQLTPE
TMEAIVEQSQRFLLPLFRFNQ

3D structure

• PDB: 6sjf A conformational switch in response to Chi converts RecBCD from phage destruction to DNA repair.
• Chain: C
• Resolution: 3.9 Å

Enzymatic activity

• Enzyme Commision number: 3.1.11.5: exodeoxyribonuclease V.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	C	R846 L875 Y878 R968 S970 L971 N1078 M1079 M1080 V1081 R1082	R846 L875 Y878 R968 S970 L971 N1078 M1079 M1080 V1081 R1082

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003678 DNA helicase activity
• GO:0004386 helicase activity
• GO:0004527 exonuclease activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008854 exodeoxyribonuclease V activity

Biological Process

• GO:0000724 double-strand break repair via homologous recombination
• GO:0000725 recombinational repair
• GO:0006281 DNA repair
• GO:0006310 DNA recombination
• GO:0009314 response to radiation
• GO:0032508 DNA duplex unwinding
• GO:0044355 clearance of foreign intracellular DNA

Cellular Component

• GO:0009338 exodeoxyribonuclease V complex

External links

• PDB: RCSB:6sjf, PDBe:6sjf, PDBj:6sjf
• PDBsum: 6sjf
• PubMed: 31907455
• UniProt: P07648|RECC_ECOLI RecBCD enzyme subunit RecC (Gene Name=recC)