Structure of PDB 6sjb Chain C

Receptor sequence

>6sjbC (length=1121) Species: 562 (Escherichia coli)

MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQWLQMTL
SQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLP
QLLEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWE
TGHLVEGLGEAQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESA
TTCPPGLPSRVFICGISALPPVYLQALQALGKHIEIHLLFTNPCRYYWGD
IKDPAYLAKLLTRQRRHSFEDRELPLFRDSENAGQLFNSDGEQDVGNPLL
ASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNLLHNIQSDILELENRAV
AGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDRLLAMLEED
PTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNES
GIRWGIDDDNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPY
DESSGLIAELVGHLASLLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFL
PDAETEAAMTLIEQQWQAIIAEGLGAQYGDAVPLSLLRDELAQRLDQERI
SQRFLAGPVNICTLMPMRSIPFKVVCLLGMNDGVYPRQLAPLGFDLMSQK
PKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNSERFPSVLVQE
LIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGERQ
SYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQ
MRLQVNFRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRR
FRAAGDLPYGAFGEIFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGV
QITGWLPQVQPDGLLRWRPSLLSVAQGMQLWLEHLVYCASGGNGESRLFL
RKDGEWRFPPLAAEQALHYLSQLIEGYREGMSAPLLVLPESGGAWLKTCY
DAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDIWYQRLWRQLTPE
TMEAIVEQSQRFLLPLFRFNQ

3D structure

• PDB: 6sjb A conformational switch in response to Chi converts RecBCD from phage destruction to DNA repair.
• Chain: C
• Resolution: 3.7 Å

Enzymatic activity

• Enzyme Commision number: 3.1.11.5: exodeoxyribonuclease V.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	C	F62 L64 P65 A66 W70 K82 S84 D133 D136 Q137 V140 Y141 R186 R649 I650 Q652 R653 T663 M665 P666 R687 R706 R708 D712 R846 Q850 L875 Y878 R968 S970 L971 N1078 M1080 V1081 R1082	F62 L64 P65 A66 W70 K82 S84 D133 D136 Q137 V140 Y141 R186 R649 I650 Q652 R653 T663 M665 P666 R687 R706 R708 D712 R846 Q850 L875 Y878 R968 S970 L971 N1078 M1080 V1081 R1082

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003678 DNA helicase activity
• GO:0004386 helicase activity
• GO:0004527 exonuclease activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008854 exodeoxyribonuclease V activity

Biological Process

• GO:0000724 double-strand break repair via homologous recombination
• GO:0000725 recombinational repair
• GO:0006281 DNA repair
• GO:0006310 DNA recombination
• GO:0009314 response to radiation
• GO:0032508 DNA duplex unwinding
• GO:0044355 clearance of foreign intracellular DNA

Cellular Component

• GO:0009338 exodeoxyribonuclease V complex

External links

• PDB: RCSB:6sjb, PDBe:6sjb, PDBj:6sjb
• PDBsum: 6sjb
• PubMed: 31907455
• UniProt: P07648|RECC_ECOLI RecBCD enzyme subunit RecC (Gene Name=recC)