Structure of PDB 6rj9 Chain C

Receptor sequence

>6rj9C (length=836) Species: 322159 (Streptococcus thermophilus LMD-9)

DLVLGLDIGIGSVGVGILNKVTGEIIHKNSRIFPAAQAENNLVRRTNRQG
RRLTRRKKHRRVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDELSN
EELFIALKNMVKHRGISYLDDYAQIVKENSKQLETKTPGQIQLERYQTYG
QLRGDFTVEKDGKKHRLINVFPTSAYRSEALRILQTQQEFNPQITDEFIN
RYLEILTGKRKYYHGPGNEKSRTDYGRYRTSGETLDNIFGILIGKCTFYP
DEFRAAKASYTAQEFNLLNDLNNLTVPKKLSKEQKNQIINYVKNEKAMGP
AKLFKYIAKLLSCDVADIKGYRIDKSGKAEIHTFEAYRKMKTLETLDIEQ
MDRETLDKLAYVLTLNTEREGIQEALEHEFADGSFSQKQVDELVQFRKAN
SSIFGKGWHNFSVKLMMELIPELYETSEEQMTILTRLGKQKTKYIDEKLL
TEEIYNPVVAKSVRQAIKIVNAAIKEYGDFDNIVIEASRVVLNALQEHFR
AHKIDTKVSVVRGQFTSQLRRHWGHHHAVDALIIAASSILFSYQVDSKFN
RKISDATIYATRQAKVGKDKADETYVLGKIKDIYTQDGYDAFMKIYKKDK
SKFLMYRHDPQTFEKVIEPILENPFLKYKEEHGYIRKYSKKGNGPEIKSL
KYYDSKLGNHIDITPKDSNNKVVLQSVSPWRADVYFNKTTGKYEILGLKY
ADLQFEKGTGTYKISQEKYNDIKKKEGVDSDSEFKFTLYKNDLLLVKDTE
TKEQQLFRFLSRTMPKQKHYVELKPYDKQKFEGGEALIKVLGNVANSGQC
KKGLGKSNISIYKVRTDVLGNQHIIKNEGDKPKLDF

3D structure

• PDB: 6rj9 Cas9 Allosteric Inhibition by the Anti-CRISPR Protein AcrIIA6.
• Chain: C
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	C	N43 R46 R47 N49 R50 R53 R54 R57 R58 K60 H61 R63 R68 I84 N85 Y89 K110 R116 G117 L165 R166 G167 K222 R223 Y225 P229 N231 K233 S234 Y238 K270 R338 K341 H348 H425 N426 F427 Q446 M447 K464 K466 Y467 K484 K491 V814 S816 K817 N819 R820 K821 I822 I827 A829 R831 K848 L873 M874 H877 D878 K922 Y923 S924 P930 E931 K933 S934 K936 D952 F990 K992 Y997 H1108	N41 R44 R45 N47 R48 R51 R52 R55 R56 K58 H59 R61 R66 I82 N83 Y87 K108 R114 G115 L152 R153 G154 K209 R210 Y212 P216 N218 K220 S221 Y225 K257 R322 K325 H332 H409 N410 F411 Q430 M431 K441 K443 Y444 K461 K468 V545 S547 K548 N550 R551 K552 I553 I558 A560 R562 K579 L604 M605 H608 D609 K637 Y638 S639 P645 E646 K648 S649 K651 D667 F705 K707 Y712 H823
BS02	dna	C	Y225 R267 N286 I339 E445 M447 Y478	Y212 R254 N273 I323 E429 M431 Y455

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:6rj9, PDBe:6rj9, PDBj:6rj9
• PDBsum: 6rj9
• PubMed: 31604602
• UniProt: Q03LF7|CAS9A_STRTD CRISPR-associated endonuclease Cas9 1 (Gene Name=cas9-1)