Structure of PDB 6qs7 Chain C

Receptor sequence

>6qs7C (length=630) Species: 562 (Escherichia coli)

QALKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEP
GVGKTAIVEGLAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEER
LKGVLNDLAKQEGNVILFIDALHTMVGAGKADGAMDAGNMLKPALARGEL
HCVGATTLDEYRQYIEKDAALERRFQKVFVAEPSVEDTIAILRGLKERYE
LHHHVQITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASSIRMQIDSK
PEELDRLDRRIIQLKLEQQALMLDMLNEELSDKERQYSELEEEWKAEKLR
NKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQELHHRVIGQNEAVD
AVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFDSDEA
MVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDA
VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQER
FGELDYAHMKELVLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQ
LKRLYKRLEERGYEIHISDEALKLLSENGYDPVYGARPLKRAIQQQIENP
LAQQILSGELVPGKVIRLEVNEDRIVAVQH

3D structure

• PDB: 6qs7 Two-Step Activation Mechanism of the ClpB Disaggregase for Sequential Substrate Threading by the Main ATPase Motor.
• Chain: C
• Resolution: 3.8 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	C	Y251 R252 H641 G652 Y653	Y93 R94 H413 G424 Y425
BS02	AGS	C	I181 P208 G209 G211 K212 T213 A214 I349 L353 I391	I23 P50 G51 G53 K54 T55 A56 I191 L195 I233
BS03	AGS	C	R569 I571 G608 V609 G610 K611 T612 E613 I774 R815 K818	R341 I343 G380 V381 G382 K383 T384 E385 I546 R587 K590
BS04	MG	C	T612 D677	T384 D449
BS05	AGS	C	R331 R332	R173 R174
BS06	AGS	C	E752 R756	E524 R528

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity
• GO:0042802 identical protein binding

Biological Process

• GO:0009408 response to heat
• GO:0034605 cellular response to heat
• GO:0042026 protein refolding

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0016020 membrane

External links

• PDB: RCSB:6qs7, PDBe:6qs7, PDBj:6qs7
• PDBsum: 6qs7
• PubMed: 31216466
• UniProt: P63284|CLPB_ECOLI Chaperone protein ClpB (Gene Name=clpB)