Structure of PDB 6p7e Chain C

Receptor sequence

>6p7eC (length=672) Species: 10760 (Escherichia phage T7) [Search protein sequence]

MIVSAIAANALLESVTKFHCGVIYDYSTAEYVSYRPSDFGAYLDALEAEV
ARGGLIVFHNGHKYDVPALTKLAKLQLNREFHLPRENCIDTLVLSRLIHS
NLKDTDMGLLRSGKLPGKRFGSHALEAWGYRLGEMKGEYKDDFKRMLEEQ
GEEYVDGMEWWNFNEEMMDYNVQDVVVTKALLEKLLSDKHYFPPEIDFTD
VGYTTFWSESLEAVDIEHRAAWLLAKQERNGFPFDTKAIEELYVELAARR
SELLRKLTETFGSWYQPKGGTEMFCHPRTGKPLPKYPRDTREYVAGAPYT
PVEHVVFNPSSRDHIQKKLQEAGWVPTKYTDKGAPVVDDEVLEGVRVDDP
EKQAAIDLIKEYLMIQKRIGQSAEGDKAWLRYVAEDGKIHGSVNPNGAVT
GRATHAFPNLAQIPGVRSPYGEQCRAAFGAEHHLDGITGKPWVQAGIDAS
GLELRCLAHFMARFDNGEYAHEILNGDIHTKNQIAAELPTRDNAKTFIYG
FLYGAGDEKIGQIVGAGKERGKELKKKFLENTPAIAALRESIQQTLVESS
QWQVKWKRRWIKGLDGRKVHVRSPHAALNTLLQSAGALICKLWIIKTEEM
LVEKGLKHGWDGDFAYMAWVHDEIQVGCRTEEIAQVVIETAQEAMRWVGD
HWNFRCLLDTEGKMGPNWAICH

3D structure

PDB

6p7e Combined Solution and Crystal Methods Reveal the Electrostatic Tethers That Provide a Flexible Platform for Replication Activities in the Bacteriophage T7 Replisome.

Chain

Resolution

3.001 Å

3D
structure

[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]

Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.
3.1.11.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	C	P284 K285 Y320 R339 T357 K359 V363 V364 D365 K394 R429 A438 Q439 I440 G442 H653	P284 K285 Y293 R312 T330 K332 V336 V337 D338 K367 R402 A411 Q412 I413 G415 H621
BS02	dna	C	R318 S337 S338 G402 K404 T431 H432 A433 Q439 Y526 G527 Y530 A532 G533 K536 W579 R604 H607 N611 Q615	R291 S310 S311 G375 K377 T404 H405 A406 Q412 Y499 G500 Y503 A505 G506 K509 W552 R572 H575 N579 Q583
BS03	peptide	C	R490 I569 I593 R687	R463 I542 I561 R655
BS04	TTP	C	A7 A8 A10 Y64 W160	A7 A8 A10 Y64 W160
BS05	MG	C	D475 A476 D654	D448 A449 D622
BS06	MG	C	D475 D654	D448 D622
BS07	TTP	C	D475 A476 G478 E480 H506 R518 K522 T523 Y526 D654	D448 A449 G451 E453 H479 R491 K495 T496 Y499 D622

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0004527	exonuclease activity
GO:0004529	DNA exonuclease activity
GO:0005515	protein binding
GO:0008408	3'-5' exonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication
GO:0006302	double-strand break repair
GO:0039693	viral DNA genome replication
GO:0090592	DNA synthesis involved in DNA replication

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:6p7e, PDBe:6p7e, PDBj:6p7e
PDBsum	6p7e
PubMed	31659895
UniProt	P00581\|DPOL_BPT7 DNA-directed DNA polymerase (Gene Name=5)

[Back to BioLiP]