Structure of PDB 6me0 Chain C

Receptor sequence

>6me0C (length=414) Species: 197221 (Thermosynechococcus vestitus BP-1)

DWAKANREVKRLQVRIAKAVKEGRWGKVKALQWLLTHSFYGKALAVKRVT
DNSGSKTPGVDGITWSTQEQKAQAIKSLRRRGYKPQPLRRVYIPKASGKQ
RPLGIPTTKDRAMQALYALALEPVAETTADRNSYGFRQGRCTADAAGQCF
TVLGRSDCAKYILDADITGCFDNISHEWLLDNIPLDKEVLRKWLKSGFVW
KQQLFPGVISPMLANMTLDGMEELLKKHLRKQKVNLIRYADDFVVTGESK
ETLEKVTTVIQEFLKERGLTLSEEKTKVVHIEEGFDFLGWNIRKYGEKLL
IKPAKKNIKAFHKKIRDALKELRTATQEAVIDTLNPIIKGWANYHRNQVS
KRIFNRADDNIWHKLWRWAKRRHPNKPARWTKNKYFIKIGNRHWVFGTWR
SRYLIKAGDTRIQR

3D structure

• PDB: 6me0 Cryo-EM Structures of a Group II Intron Reverse Splicing into DNA.
• Chain: C
• Resolution: 3.6 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	C	R37 R41 K44 R50 W51 G52 K53 W59 L60 S64 F65 Y66 R106 R107 G108 K110 Q112 P113 R115 R116 Y118 A122 S123 G124 E214 K221 W226 Q228 E308 R327 Y329 K336 K340 K343 K347 R350 K354 R386 R390 D393 H397 R401 R413	R11 R15 K18 R24 W25 G26 K27 W33 L34 S38 F39 Y40 R80 R81 G82 K84 Q86 P87 R89 R90 Y92 A96 S97 G98 E188 K195 W200 Q202 E274 R293 Y295 K302 K306 K309 K313 R316 K320 R352 R356 D359 H363 R367 R379
BS02	dna	C	L334 K385 N389 D393 W396 W400 P411 A412 R413 K416 N425 R426 W428	L300 K351 N355 D359 W362 W366 P377 A378 R379 K382 N391 R392 W394

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003964 RNA-directed DNA polymerase activity
• GO:0004519 endonuclease activity
• GO:0008270 zinc ion binding

Biological Process

• GO:0006278 RNA-templated DNA biosynthetic process
• GO:0006974 DNA damage response

External links

• PDB: RCSB:6me0, PDBe:6me0, PDBj:6me0
• PDBsum: 6me0
• PubMed: 31348888
• UniProt: Q8DMK2