Structure of PDB 6kj6 Chain C

Receptor sequence
>6kj6C (length=1327) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
VYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEA
AFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLR
LVIYIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDKG
KTHSKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALN
YTTEQILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVE
KGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAAN
MELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRLSALV
EIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLREEI
EGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQF
RVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQF
MDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETP
EGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNY
VIAQANSNLDEEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVG
ASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGV
TAVAKRGGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTC
INQMPCVSLGEPVERGDVLADGPSTDLGELALGQNMRVAFMPWNGYNFED
SILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPNVGEAALSK
LDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEEL
QILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQL
AEQYDELKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGD
KMAGRHGNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILE
THLGMAAKGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDLSTF
SDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDLPTSGQIRLYD
GRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQ
FGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQ
MEPGMPESFNVLLKEIRSLGINIELED
3D structure
PDB6kj6 Crl activates transcription by stabilizing active conformation of the master stress transcription initiation factor.
ChainC
Resolution3.8 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna C N139 R143 S508 F514 G1261 R1269 M1273 N127 R131 S494 F500 G1247 R1255 M1259
BS02 dna C R151 R175 W183 R200 R201 R371 I445 R451 R542 R139 R161 W169 R186 R187 R357 I431 R437 R528
BS03 rna C Q510 N568 Q688 K1073 H1237 Q496 N554 Q674 K1059 H1223
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0005515 protein binding
GO:0016779 nucleotidyltransferase activity
GO:0032549 ribonucleoside binding
GO:0034062 5'-3' RNA polymerase activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006879 intracellular iron ion homeostasis
GO:0009408 response to heat
GO:0031564 transcription antitermination
GO:0032784 regulation of DNA-templated transcription elongation
GO:0036460 cellular response to cell envelope stress
GO:0042128 nitrate assimilation
GO:0044780 bacterial-type flagellum assembly
GO:0046677 response to antibiotic
GO:0048870 cell motility
GO:0071973 bacterial-type flagellum-dependent cell motility
GO:0090605 submerged biofilm formation
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0000428 DNA-directed RNA polymerase complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0008023 transcription elongation factor complex
GO:0016020 membrane

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:6kj6, PDBe:6kj6, PDBj:6kj6
PDBsum6kj6
PubMed31846423
UniProtP0A8V2|RPOB_ECOLI DNA-directed RNA polymerase subunit beta (Gene Name=rpoB)

[Back to BioLiP]