Structure of PDB 6jzs Chain C

Receptor sequence

>6jzsC (length=453) Species: 1404 (Priestia megaterium)

KEMPQPKTFGELKNLPLLNTDKPVQALMKIADELGEIFKFEAPGRVTRYL
SSQRLIKEACDESRFDKNLSQALKFVRDFAGDGLFTSWTHEKNWKKAHNI
LLPSFSQQAMKGYHAMMVDIAVQLVQKWERLNADEHIEVPEDMTRLTLDT
IGLCGFNYRFNSFYRDQPHPFITSMVRALDEAMNKLQRANPDDPAYDENK
RQFQEDIKVMNDLVDKIIADRKASGEQSDDLLTHMLNGKDPETGEPLDDE
NIRYQIITFLIAGHETTSGLLSFALYFLVKNPHVLQKAAEEAARVLVDPV
PSYKQVKQLKYVGMVLNEALRLWPTAPAFSLYAKEDTVLGGEYPLEKGDE
LMVLIPQLHRDKTIWGDDVEEFRPERFENPSAIPQHAFKPFGNGQRACIG
QQFALHEATLVLGMMLKHFDFEDHTNYELDIKETLTLKPEGFVVKAKSKK
IPL

3D structure

• PDB: 6jzs Crystals in Minutes: Instant On-Site Microcrystallisation of Various Flavours of the CYP102A1 (P450BM3) Haem Domain.
• Chain: C
• Resolution: 1.68 Å

Enzymatic activity

• Enzyme Commision number: 1.14.14.1: unspecific monooxygenase.
  1.6.2.4: NADPH--hemoprotein reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	0PY	C	F87 A264 T268	F85 A262 T266
BS02	0PY	C	F158 Y160 F162 H171 F173 L215	F156 Y158 F160 H169 F171 L213
BS03	WAA	C	L20 P25 A44 R47 Y51 S72 Q73 A74 L75 F87 L188 L437	L18 P23 A42 R45 Y49 S70 Q71 A72 L73 F85 L186 L435
BS04	MNH	C	K69 L86 F87 W96 A264 T269 F331 P392 F393 R398 C400 I401 G402	K67 L84 F85 W94 A262 T267 F329 P390 F391 R396 C398 I399 G400

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0005506 iron ion binding
• GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
• GO:0020037 heme binding

External links

• PDB: RCSB:6jzs, PDBe:6jzs, PDBj:6jzs
• PDBsum: 6jzs
• PubMed: 32157795
• UniProt: P14779|CPXB_PRIM2 Bifunctional cytochrome P450/NADPH--P450 reductase (Gene Name=cyp102A1)