Structure of PDB 6ech Chain C

Receptor sequence
>6echC (length=528) Species: 10116 (Rattus norvegicus) [Search protein sequence]
TAFFQQQQLPAAMADTFLEHLCLLDIDSQPVAARSTSIIATIGPASRSVD
RLKEMIKAGMNIARLNFSHGSHEYHAESIANIREATESFATSPLSYRPVA
IALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKFQTRGDAKTVWV
DYHNITRVVAVGGRIYIDDGLISLVVQKIGPEGLVTEVEHGGILGSRKGV
NLPNTEVDLPGLSEQDLLDLRFGVQHNVDIIFASFVRKASDVLAVRDALG
PEGQNIKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLA
QKMMIGRCNLAGKPVVCATQMLESMITKARPTRAETSDVANAVLDGADCI
MLSGETAKGSFPVEAVMMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPT
EVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSQYRPRAAVIAVTRSAQA
ARQVHLSRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLV
IVVTGWRPGSGYTNIMRVLSVSHHHHHH
3D structure
PDB6ech Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production.
ChainC
Resolution2.19 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R85 R132 K282 T340
Catalytic site (residue number reindexed from 1) R64 R111 K261 T319
Enzyme Commision number 2.7.1.40: pyruvate kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FBP C L443 T444 K445 T446 S449 R501 G526 R528 G530 S531 G532 Y533 T534 L422 T423 K424 T425 S428 R480 G505 R507 G509 S510 G511 Y512 T513
BS02 MG C E284 D308 E263 D287
BS03 MG C N87 S89 D125 T126 N66 S68 D104 T105
BS04 OXL C K282 E284 A305 G307 D308 T340 K261 E263 A284 G286 D287 T319
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003824 catalytic activity
GO:0004743 pyruvate kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0030955 potassium ion binding
GO:0046872 metal ion binding
GO:0048029 monosaccharide binding
Biological Process
GO:0001666 response to hypoxia
GO:0006096 glycolytic process
GO:0007584 response to nutrient
GO:0009749 response to glucose
GO:0010038 response to metal ion
GO:0016310 phosphorylation
GO:0032869 cellular response to insulin stimulus
GO:0033198 response to ATP
GO:0042866 pyruvate biosynthetic process
GO:0051591 response to cAMP
GO:0051707 response to other organism
GO:0071872 cellular response to epinephrine stimulus
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:6ech, PDBe:6ech, PDBj:6ech
PDBsum6ech
PubMed31825824
UniProtP12928|KPYR_RAT Pyruvate kinase PKLR (Gene Name=Pklr)

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