Structure of PDB 6dhn Chain C

Receptor sequence

>6dhnC (length=501) Species: 9913 (Bos taurus)

ADREDDPNFFKMVEGFFDRGASIVEDKLVEDLKTRETEEQKRNRVRGILR
IIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHSQHRTPCKGGIRYSTDVS
VDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTDNELEKITRRFTM
ELAKKGFIGPGVDVPAPDMSTGEREMSWIADTYASTIGHYDINAHACVTG
KPISQGGIHGRISATGRGVFHGIENFINEASYMSILGMTPGFGDKTFAVQ
GFGNVGLHSMRYLHRFGAKCVAVGESDGSIWNPDGIDPKELEDFKLQHGT
ILGFPKAKIYEGSILEVDCDILIPAASEKQLTKSNAPRVKAKIIAEGANG
PTTPEADKIFLERNIMVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFK
YERDSNYHLLMSVQESLERKFGKHGGTIPIVPTAEFQDRISGASEKDIVH
SGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFRVYNEAGVTF
T

3D structure

• PDB: 6dhn A novel mechanism of V-type zinc inhibition of glutamate dehydrogenase results from disruption of subunit interactions necessary for efficient catalysis.
• Chain: C
• Resolution: 3.3 Å

Enzymatic activity

• Enzyme Commision number: 1.4.1.3: glutamate dehydrogenase [NAD(P)(+)].

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAI	C	H85 R86 T87 D119 P121 K488	H85 R86 T87 D119 P121 K488
BS02	GLU	C	K90 M111 K114 K126 A166 D168 R211 G377 V378 S381	K90 M111 K114 K126 A166 D168 R211 G377 V378 S381
BS03	GTP	C	R261 Y262 R265 E292	R261 Y262 R265 E292
BS04	NAI	C	H195 Q205 K387 N388 H391 V392 S393	H195 Q205 K387 N388 H391 V392 S393
BS05	NAI	C	R94 D168 M169 R211 T215 Q250 G253 N254 V255 E275 S276 A326 A348 N349 N374	R94 D168 M169 R211 T215 Q250 G253 N254 V255 E275 S276 A326 A348 N349 N374

Gene Ontology

Molecular Function

• GO:0004352 glutamate dehydrogenase (NAD+) activity
• GO:0004353 glutamate dehydrogenase [NAD(P)+] activity
• GO:0004354 glutamate dehydrogenase (NADP+) activity
• GO:0005524 ATP binding
• GO:0005525 GTP binding
• GO:0016491 oxidoreductase activity
• GO:0016639 oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor
• GO:0042802 identical protein binding

Biological Process

• GO:0006520 amino acid metabolic process
• GO:0006538 glutamate catabolic process
• GO:0006541 glutamine metabolic process
• GO:0072350 tricarboxylic acid metabolic process

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0005783 endoplasmic reticulum

External links

• PDB: RCSB:6dhn, PDBe:6dhn, PDBj:6dhn
• PDBsum: 6dhn
• PubMed: 21749647
• UniProt: P00366|DHE3_BOVIN Glutamate dehydrogenase 1, mitochondrial (Gene Name=GLUD1)