Structure of PDB 6dhm Chain C

Receptor sequence

>6dhmC (length=495) Species: 9913 (Bos taurus)

ADREDDPNFFKMVEGFFDRGASIVEDKLVEDLKTRETEEQKRNRVRGILR
IIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHSQHRTPCKGGIRYSTDVS
VDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTDNELEKITRRFTM
ELAKKGFIGPGVDVPAPDMSTGEREMSWIADTYASTIGHYDINAHACVTG
KPISQGGIHGRISATGRGVFHGIENFINEASYMSILGMTPGFGDKTFAVQ
GFGNVGLHSMRYLHRFGAKCVAVGESDGSIWNPDGIDPKELEDFKLQHGT
ILGFPKAKIYEGSILEVDCDILIPAASEKQLTKSNAPRVKAKIIAEGANG
PTTPEADKIFLERNIMVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFK
YERDSNYHLLMSVQESLERKFGKHGGTIPIVPTAEFQDRISGASEKDIVH
SGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFRVYNE

3D structure

• PDB: 6dhm A novel mechanism of V-type zinc inhibition of glutamate dehydrogenase results from disruption of subunit interactions necessary for efficient catalysis.
• Chain: C
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 1.4.1.3: glutamate dehydrogenase [NAD(P)(+)].

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLU	C	K90 G91 M111 K114 K126 A166 N349 V378 S381	K90 G91 M111 K114 K126 A166 N349 V378 S381
BS02	NDP	C	R94 D168 M169 S170 R211 G251 G253 N254 V255 E275 S276 A326 Q330 N349 N374	R94 D168 M169 S170 R211 G251 G253 N254 V255 E275 S276 A326 Q330 N349 N374
BS03	GTP	C	H209 S213 R261 Y262 R265 K289 E292 H450	H209 S213 R261 Y262 R265 K289 E292 H450
BS04	ZN	C	H209 H450	H209 H450

Gene Ontology

Molecular Function

• GO:0004352 glutamate dehydrogenase (NAD+) activity
• GO:0004353 glutamate dehydrogenase [NAD(P)+] activity
• GO:0004354 glutamate dehydrogenase (NADP+) activity
• GO:0005524 ATP binding
• GO:0005525 GTP binding
• GO:0016491 oxidoreductase activity
• GO:0016639 oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor
• GO:0042802 identical protein binding

Biological Process

• GO:0006520 amino acid metabolic process
• GO:0006538 glutamate catabolic process
• GO:0006541 glutamine metabolic process
• GO:0072350 tricarboxylic acid metabolic process

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0005783 endoplasmic reticulum

External links

• PDB: RCSB:6dhm, PDBe:6dhm, PDBj:6dhm
• PDBsum: 6dhm
• PubMed: 21749647
• UniProt: P00366|DHE3_BOVIN Glutamate dehydrogenase 1, mitochondrial (Gene Name=GLUD1)