Structure of PDB 6cip Chain C

Receptor sequence

>6cipC (length=1163) Species: 264732 (Moorella thermoacetica ATCC 39073)

PKQTLDGNTAAAHVAYAMSEVATIYPITPSSPMAEIADEWAAHGRKNIFG
KTLQVAEMQSEAGAAGAVHGSLAAGALTTTFTASQGLLLMIPNMYKIAGE
LLPCVFHVAARALSTHALSIFGDHADVMAARQTGFAMLSSASVQEVMDLA
LVAHLATLKARVPFVHFFDGFRTSHEVQKIDVIEYEDMAKLVDWDAIRAF
RQRALNPEHPHQRGTAQNPDIYFQSREAANPYYLATPGIVAQVMEQVAGL
TGRHYHLFDYAGAPDAERVIVSMGSSCEVIEETVNYLVEKGEKVGLIKVR
LFRPFSAEHFLKVLPASVKRIAVLDRTKEPGSLGEPLYEDVQTVLAEHGK
NILVVGGRYGLGSKEFNPSMVKAVFDNLAATTPKNKFTVGITDDVTHTSL
EIKEHIDTSPKGTFRCKFFGLGSDGTVGANKNSIKIIGDHTDMYAQGYFV
YDSKKSGGVTISHLRFGKQPIQSAYLIDQADLIACHNPSYVGRYNLLEGI
KPGGIFLLNSTWSAEEMDSRLPADMKRTIATKKLKFYNIDAVKIAQEIGL
GSRINVIMQTAFFKIANVIPVDEAIKYIKDSIVKTYGKKGDKILNMNFAA
VDRALEALEEIKYPASWADAVDTEEPEFIQKVLRPINALKGDELPVSTFT
PDGVFPVGTTKYEKRGIAVNIPQWQPENCIQCNQCSLVCPHAAIRPYLAK
PADLAGAPETFVTKDAIGKEAAGLKFRIQVSPLDCTGCGNCADVCPAKVK
ALTMVPLEEVTAVEEANYNFAEQLPEVKVNFNPATVKGSQFRQPLLEFSG
ACAGCGETPYVKLVTQLFGDRMIIANATGCSSIWGGSAPACPYTVNRQGH
GPAWASSLFEDNAEFGYGMALAVAKRQDELATAISKALEAPVSAAFKAAC
EGWLAGKDDADRSREYGDRIKALLPGEISQASGEVKDLLLDIDRQKDYLT
KKSIWIIGGDGWAYDIGYGGLDHVLASGANVNVLVLDTEVYSNTGGQSSK
ATQTGAVARFAAGGKFTKKKDLGLMAMSYGYVYVASVAMGASHSQLMKAL
IEAEKYDGPSLIIAYAPCINHGINMTYSQREAKKAVEAGYWPLYRYNPQL
AQEGKNPFILDYKTPTASFRDFLMGEIRYTSLKKQFPEKAEQLFAKAEAD
AKARLEQYKKLAE

3D structure

• PDB: 6cip Binding site for coenzyme A revealed in the structure of pyruvate:ferredoxin oxidoreductase fromMoorella thermoacetica.
• Chain: C
• Resolution: 3.189 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T29 E62 R112 N1000
• Catalytic site (residue number reindexed from 1): T28 E61 R111 N993
• Enzyme Commision number: 1.2.7.1: pyruvate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	C	C686 I687 Q688 C689 N690 C692 C752 P753 L759	C679 I680 Q681 C682 N683 C685 C745 P746 L752
BS02	SF4	C	P679 C696 I701 C742 T743 G744 C745 G746 N747 C748	P672 C689 I694 C735 T736 G737 C738 G739 N740 C741
BS03	SF4	C	C809 C812 E814 C837 S999 C1075 I1076	C802 C805 E807 C830 S992 C1068 I1069
BS04	HTL	C	I28 E62 Q86 R112 T835 G836 C837 F866 D967 G968 V997 Y998 S999 N1000 T1001	I27 E61 Q85 R111 T828 G829 C830 F859 D960 G961 V990 Y991 S992 N993 T994
BS05	MG	C	D967 T995 V997	D960 T988 V990

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0005506 iron ion binding
• GO:0016491 oxidoreductase activity
• GO:0016903 oxidoreductase activity, acting on the aldehyde or oxo group of donors
• GO:0019164 pyruvate synthase activity
• GO:0030976 thiamine pyrophosphate binding
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006086 acetyl-CoA biosynthetic process from pyruvate
• GO:0006979 response to oxidative stress
• GO:0022900 electron transport chain

External links

• PDB: RCSB:6cip, PDBe:6cip, PDBj:6cip
• PDBsum: 6cip
• PubMed: 29581263
• UniProt: Q2RMD6|PFOR_MOOTA Pyruvate:ferredoxin oxidoreductase (Gene Name=Moth_0064)