Structure of PDB 6c71 Chain C

Receptor sequence

>6c71C (length=440) Species: 1042876 (Pseudomonas putida S16)

GGFDYDVVVVGGGFAGATAARECGLQGYRTLLLEARSRLGGRTFTSRFAG
QEIEFGGAWVHWLQPHVWAEMQRYGLGVVEDPLTNLDKTLIMYNDGSVES
ISPDEFGKNIRIAFEKLCHDAWEVFPRPHEPMFTERARELDKSSVLDRIK
TLGLSRLQQAQINSYMALYAGETTDKFGLPGVLKLFACGGWNYDAFMDTE
THYRIQGGTIGLINAMLTDSGAEVRMSVPVTAVEQVNGGVKIKTDDDEII
TAGVVVMTVPLNTYKHIGFTPALSKGKQRFIKEGQLSKGAKLYVHVKQNL
GRVFAFADEQQPLNWVQTHDYSDELGTILSITIARKETIDVNDRDAVTRE
VQKMFPGVEVLGTAAYDWTADPFSLGAWAAYGVGQLSRLKDLQAAEGRIL
FAGAETSNGWHANIDGAVESGLRAGREVKQLLSLEHHHHH

3D structure

• PDB: 6c71 Crystallography Coupled with Kinetic Analysis Provides Mechanistic Underpinnings of a Nicotine-Degrading Enzyme.
• Chain: C
• Resolution: 2.649 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H110
• Catalytic site (residue number reindexed from 1): H61
• Enzyme Commision number: 1.4.2.2: nicotine dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NCT	C	L217 Y218 E249 T250 W364 T381 W427 A461	L168 Y169 E200 T201 W315 T332 W378 A412
BS02	FAD	C	V59 G60 G62 A64 E83 A84 R85 G90 R91 T92 G105 G106 A107 W108 V279 V308 P309 I316 W417 F422 W427 G452 A453 N462 I463 A466	V10 G11 G13 A15 E34 A35 R36 G41 R42 T43 G56 G57 A58 W59 V230 V259 P260 I267 W368 F373 W378 G403 A404 N413 I414 A417

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity

External links

• PDB: RCSB:6c71, PDBe:6c71, PDBj:6c71
• PDBsum: 6c71
• PubMed: 29812904
• UniProt: F8G0P2|NICA2_PSEP6 Nicotine dehydrogenase (Gene Name=nicA2)