Structure of PDB 5zwm Chain C

Receptor sequence
>5zwmC (length=843) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
EHTIFTQLKKNIPKTRYNRDYMLSMANIPERIINVGVIGPLHSGKTSLMD
LLVIDSHKRIPDMSKNVELGWKPLRYLDNLKQEIDRGLSIKLNGSTLLCT
DLESKSRMINFLDAPGHVNFMDETAVALAASDLVLIVIDVVEGVTFVVEQ
LIKQSIKNNVAMCFVINKLDRLILDLKLPPMDAYLKLNHIIANINSFTKG
NVFSPIDNNIIFASTKLGFTFTIKEFVSYYYAHSIPSSKIDDFTTRLWGS
VYYHKGNFRTKPFENVEKYPTFVEFILIPLYKIFSYALSMEKDKLKNLLR
SNFRVNLSQEALQYDPQPFLKHVLQLIFRQQTGLVDAITRCYQPFELFDN
KTAHLSIPGKSTPEGTLWAHVLKTVDYGGAEWSLVRIYSGLLKRGDTVRI
LDTSQSESREDDETPSCEVEEIGLLGGRYVYPVHEAHKGQIVLIKGISSA
YIKSATLYSVKSKEDMKQLKFFKPLDYITEAVFKIVLQPLLPRELPKLLD
ALNKISKYYPGVIIKVEESGEHVILGNGELYMDCLLYDLRASYAKIEIKI
SDPLTVFSESCSNSRLGEENLPGLSISVAAEPMDSKMIQDLSRNTLGKGQ
NCLDIDGIMDNPRKLSKILRTEYGWDSLASRNVWSFYNGNVLINDTLPDE
ISPELLSKYKEQIIQGFYWAVKEGPLAEEPIYGVQYKLLSISVPSDVNID
VMKSQIIPLMKKACYVGLLTAIPILLEPIYEVDITVHAPLLPIVEELMKK
RRGSRIYKTIKVAGTPLLEVRGQVPVIESAGFETDLRLSTNGLGMCQLYF
WHKIWRKVPGDVLDKDAFIPKLKPAPINSLSRDFVMKTRRRKG
3D structure
PDB5zwm Structures of the fully assembledSaccharomyces cerevisiaespliceosome before activation
ChainC
Resolution3.4 Å
3D
structure
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Enzymatic activity
Enzyme Commision number ?
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna C K111 K182 I185 H334 R405 K10 K81 I84 H233 R304
BS02 GTP C H143 S144 G145 K146 T147 S148 S190 G217 K269 D271 S315 T316 K317 H42 S43 G44 K45 T46 S47 S89 G116 K168 D170 S214 T215 K216
BS03 MG C T147 S190 T46 S89
Gene Ontology
Molecular Function
GO:0003924 GTPase activity
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0030623 U5 snRNA binding
Biological Process
GO:0000244 spliceosomal tri-snRNP complex assembly
GO:0000349 generation of catalytic spliceosome for first transesterification step
GO:0000387 spliceosomal snRNP assembly
GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
GO:0000398 mRNA splicing, via spliceosome
GO:0006397 mRNA processing
GO:0008380 RNA splicing
Cellular Component
GO:0005634 nucleus
GO:0005681 spliceosomal complex
GO:0005682 U5 snRNP
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0046540 U4/U6 x U5 tri-snRNP complex
GO:0071007 U2-type catalytic step 2 spliceosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5zwm, PDBe:5zwm, PDBj:5zwm
PDBsum5zwm
PubMed29794219
UniProtP36048|SN114_YEAST Pre-mRNA-splicing factor SNU114 (Gene Name=SNU114)

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