Structure of PDB 5utu Chain C

Receptor sequence

>5utuC (length=491) Species: 353152 (Cryptosporidium parvum Iowa II)

KMESRIKDISLAEFGLQDMEIAKTDMMGLVELQRKYRDSKPLKGARITGS
LHLTIETSVLVETLYELGAEIRWCSCNIYSTQDHAAAALVKKNIATVFAW
KNETIEDYWVCLNDAMTWRNPNKICGPNLIVDDGGDATLILHEGVKAEIE
YEKYNKIPEYLETELDENGKQLSMDLKCMYKVLKMELLKNPFRWRGMLKD
LYGVSEETTTGVLRLKIMESEGKLLLPAINVNDSVTKSKFDNTYGCRQSL
LHGLFNGCIQMLAGKKIVVLGYGEVGKGCAQGLSGVGARVIVTEIDPICA
LQASMEGYQVSVLEDVVSEADIFITATGNKDVITVEHMRKMKENAYIANI
GHFDDEIDVYGLENYPGIKVIEVKQNVHKFTFPDTQKSVILLCKGRLVNL
GCATGHPPLVMSMSFTNQVLAQMDLWKSRELVDRSKNTRFFVKKLSKELD
EYVARLHLDVLGIKLTKLTETQAKYINVSINGPYKSEDYRY

3D structure

• PDB: 5utu 2.65 Angstrom Resolution Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with SAH and NAD
• Chain: C
• Resolution: 2.65 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H53 S76 S81 D137 E211 N236 K241 D245 N246 C250 H356 H410 S418 Q422
• Catalytic site (residue number reindexed from 1): H52 S75 S80 D133 E207 N232 K237 D241 N242 C246 H352 H406 S414 Q418
• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	C	N246 C250 G277 E278 V279 E298 I299 T331 N333 I354 G355 N403	N242 C246 G273 E274 V275 E294 I295 T327 N329 I350 G351 N399
BS02	SAH	C	L52 H53 T55 E57 C77 N78 D137 T212 D245 H356 F357 L401 H410 M415	L51 H52 T54 E56 C76 N77 D133 T208 D241 H352 F353 L397 H406 M411
BS03	NAD	C	K489 Y493	K485 Y489

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5utu, PDBe:5utu, PDBj:5utu
• PDBsum: 5utu
• UniProt: Q5CPH1