Structure of PDB 5u95 Chain C

Receptor sequence

>5u95C (length=522) Species: 4097 (Nicotiana tabacum)

DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADV
ELNSRKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMAN
PLFTPAEVVKQAKASSAKIIVTQACHVNKVKDYAFENDVKIICIDSAPEG
CLHFSVLTQANEHDIPEVEIQPDDVVALPYSSTTGLPKGVMLTHKGLVTS
VAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCGLRVGAAILIMQ
KFDIVSFLELIQSYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSGA
APLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGA
CGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATART
IDKEGWLYTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNH
PNISDAAVVPMKQAGEVPVAFVVRSGSTITEDEVKDFISKQVIFYKRIKR
VFFVDAIPGKILRKDLRAKLAA

3D structure

• PDB: 5u95 Structure of the open conformation of 4-coumarate-CoA ligase from Nicotiana tabacum
• Chain: C
• Resolution: 2.25 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S189 S209 H237 T336 E337 K441 Q446 K526
• Catalytic site (residue number reindexed from 1): S181 S200 H228 T327 E328 K432 Q437 K510
• Enzyme Commision number: 6.2.1.-
  6.2.1.12: 4-coumarate--CoA ligase.
  6.2.1.34: trans-feruloyl-CoA synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	C	D434 L436	D425 L427

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0005524 ATP binding
• GO:0016207 4-coumarate-CoA ligase activity
• GO:0016405 CoA-ligase activity
• GO:0016874 ligase activity
• GO:0050563 trans-feruloyl-CoA synthase activity
• GO:0106286 (E)-caffeate-CoA ligase activity
• GO:0106290 trans-cinnamate-CoA ligase activity

Biological Process

• GO:0009611 response to wounding
• GO:0009698 phenylpropanoid metabolic process
• GO:0009753 response to jasmonic acid

External links

• PDB: RCSB:5u95, PDBe:5u95, PDBj:5u95
• PDBsum: 5u95
• UniProt: O24146|4CL2_TOBAC 4-coumarate--CoA ligase 2 (Gene Name=4CL2)