Structure of PDB 5scl Chain C

Receptor sequence

>5sclC (length=424) Species: 9606 (Homo sapiens)

ELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASR
SVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYR
PVAIALDTKGPPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAA
LGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVF
LAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGAD
CIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRD
PTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSA
QAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGD
LVIVVTGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 5scl Anthraquinone derivatives as ADP-competitive inhibitors of liver pyruvate kinase.
• Chain: C
• Resolution: 2.134 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	C	L443 T444 T445 T446 S449 W494 R501 G526 R528 P529 G530 S531 G532 Y533 T534	L324 T325 T326 T327 S330 W375 R382 G407 R409 P410 G411 S412 G413 Y414 T415
BS02	OXL	C	K282 E284 A305 G307 D308 T340	K163 E165 A186 G188 D189 T221
BS03	AZN	C	P65 N87 H90 G91 Y95 S374 G375 A378 K379	P47 N69 H72 G73 Y77 S255 G256 A259 K260
BS04	MG	C	E284 D308	E165 D189

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:5scl, PDBe:5scl, PDBj:5scl
• PDBsum: 5scl
• PubMed: 35290845
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)