Structure of PDB 5sc8 Chain C

Receptor sequence

>5sc8C (length=424) Species: 9606 (Homo sapiens)

ELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASR
SVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYR
PVAIALDTKGPPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAA
LGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVF
LAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGAD
CIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRD
PTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSA
QAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGD
LVIVVTGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 5sc8 Anthraquinone derivatives as ADP-competitive inhibitors of liver pyruvate kinase.
• Chain: C
• Resolution: 1.77 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	C	L443 T444 T445 T446 S449 W494 R501 G526 R528 P529 G530 S531 Y533 T534	L324 T325 T326 T327 S330 W375 R382 G407 R409 P410 G411 S412 Y414 T415
BS02	OXL	C	K282 E284 A305 G307 D308 T340	K163 E165 A186 G188 D189 T221
BS03	E6K	C	T62 P65 N87 H90 G91 Y95 S374 G375 A378 K379	T44 P47 N69 H72 G73 Y77 S255 G256 A259 K260
BS04	MG	C	E284 D308	E165 D189

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:5sc8, PDBe:5sc8, PDBj:5sc8
• PDBsum: 5sc8
• PubMed: 35290845
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)