Structure of PDB 5okb Chain C

Receptor sequence

>5okbC (length=474) Species: 1422 (Geobacillus stearothermophilus)

HLKPFPPEFLWGAASAAYQVEGAWNEDGKGLSVWDVFAKQPGRTFKGTNG
DVAVDHYHRYQEDVALMAEMGLKAYRFSVSWSRVFPDGNGAVNEKGLDFY
DRLIEELRNHGIEPIVTLYHWDVPQALMDAYGAWESRRIIDDFDRYAVTL
FQRFGDRVKYWVTLNEQNIFISFGYRLGLHPPGVKDMKRMYEANHIANLA
NAKVIQSFRHYVPDGKIGPSFAYSPMYPYDSRPENVLAFENAEEFQNHWW
MDVYAWGMYPQAAWNYLESQGLEPTVAPGDWELLQAAKPDFMGVNYYQTT
TVEHNPPDGVGEGVMNTTGKKGTSTSSGIPGLFKTVRNPHVDTTNWDWAI
DPVGLRIGLRRIANRYQLPILITENGLGEFDTLEPGDIVNDDYRIDYLRR
HVQEIQRAITDGVDVLGYCAWSFTDLLSWLNGYQKRYGFVYVNRDDESEK
DLRRIKKKSFYWYQRVIETNGAEL

3D structure

• PDB: 5okb Structural basis for enzyme bifunctionality - the case of Gan1D from Geobacillus stearothermophilus.
• Chain: C
• Resolution: 1.331 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R80 H124 E170 I173 N299 Y301 E378
• Catalytic site (residue number reindexed from 1): R76 H120 E166 I169 N295 Y297 E374
• Enzyme Commision number: 3.2.1.85: 6-phospho-beta-galactosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PO4	C	Q23 S432	Q19 S428

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0008422 beta-glucosidase activity
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0033920 6-phospho-beta-galactosidase activity

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0016052 carbohydrate catabolic process

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5okb, PDBe:5okb, PDBj:5okb
• PDBsum: 5okb
• PubMed: 28975708
• UniProt: W8QF82