Structure of PDB 5ng6 Chain C

Receptor sequence

>5ng6C (length=1268) Species: 401614 (Francisella tularensis subsp. novicida U112)

NASIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKDYKK
AKQIIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFK
SAKDTIKKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNG
IELFKANSDITDIDEALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSI
IYRIVDDNLPKFLENKAKYESLKDKAPEAINYEQIKKDLAEELTFDIDYK
TSEVNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFVNGENTKRKG
INEYINLYSQQINDKTLKKYKMSVLFKQILSDTDKLEDDSDVVTTMQSFY
EQIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLTDLSQQV
FDDYSVIGTAVLEYITQQIAPKNQELIAKKTEKAKYLSLETIKLALEEFN
KHRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDL
LQASAEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLV
FEECYFELANIVPLYNKIRNYITQKPYSDEKFKLNFENSTLANGWDKNKE
PDNTAILFIKDDKYYLGVMNKKNNKIFDDKAIKENKGEGYKKIVYKLLPG
ANKMLPKVFFSAKSIKFYNPSEDILRIRNHSTHTKNGSPQKGYEKFEFNI
EDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEFYREVENQGYKL
TFENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWKALFDE
RNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKKESVFE
YDLIKDKRFTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKANDVHIL
SIDRGERHLAYYTLVDGKGNIIKQDTFNIIGNDRMKTNYHDKLAAIERKD
WKKINNIKEMKEGYLSQVVHEIAKLVIEYNAIVVFEDLNFGFKRGRFKVE
KQVYQKLEKMLIEKLNYLVFKDNEFDKTGGVLRAYQLTAPFETFKKMGKQ
TGIIYYVPAGFTSKICPVTGFVNQLYPKYESVSKSQEFFSKFDKICYNLD
KGYFEFSFDYKNFAAKGKWTIASFGSRLINNWDTREVYPTKELEKLLKDY
SIEYGHGECIKAAICGESDKKFFAKLTSVLNTILQMRNSKTGTELDYLIS
PVADVNGNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNNQEGKK
LNLVIKNEEYFEFVQNRN

3D structure

• PDB: 5ng6 Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a.
• Chain: C
• Resolution: 3.342 Å

Enzymatic activity

• Enzyme Commision number: 3.1.21.1: deoxyribonuclease I.
  4.6.1.22: Bacillus subtilis ribonuclease.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	C	S14 K15 T16 R18 F19 G181 N185 K589 Y591 K595 N790 K791 S794 Y796 S797 G799 R800 N802 L803 H804 F831 R833 I849 A850 N851 K852 N853 N856 S861 V862 F863 Y865 L867 I868 K869 D870 K871 R872 F879 H881 M949 Y953 K956 G983 S986 Q987 H990 K1034 K1041	S15 K16 T17 R19 F20 G182 N186 K575 Y577 K581 N776 K777 S780 Y782 S783 G785 R786 N788 L789 H790 F817 R819 I835 A836 N837 K838 N839 N842 S847 V848 F849 Y851 L853 I854 K855 D856 K857 R858 F865 H867 M935 Y939 K942 G963 S966 Q967 H970 K1014 K1021

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004530 deoxyribonuclease I activity
• GO:0016829 lyase activity

Biological Process

• GO:0006259 DNA metabolic process
• GO:0051607 defense response to virus

External links

• PDB: RCSB:5ng6, PDBe:5ng6, PDBj:5ng6
• PDBsum: 5ng6
• PubMed: 28431230
• UniProt: A0Q7Q2|CS12A_FRATN CRISPR-associated endonuclease Cas12a (Gene Name=cas12a)