Structure of PDB 5mps Chain C

Receptor sequence

>5mpsC (length=874) Species: 4932 (Saccharomyces cerevisiae)

EVEVLMETKNTQSPQTPLVEPVTERTKLQEHTIFTQLKKNIPKTRYNRDY
MLSMANIPERIINVGVIGPLHSGKTSLMDLLVIDSHKRIPDMSKNVELGW
KPLRYLDNLKQEIDRGLSIKLNGSTLLCTDLESKSRMINFLDAPGHVNFM
DETAVALAASDLVLIVIDVVEGVTFVVEQLIKQSIKNNVAMCFVINKLDR
LILDLKLPPMDAYLKLNHIIANINSFTKGNVFSPIDNNIIFASTKLGFTF
TIKEFVSYYYAHSIPSSKIDDFTTRLWGSVYYHKGNFRTKPFENVEKYPT
FVEFILIPLYKIFSYALSMEKDKLKNLLRSNFRVNLSQEALQYDPQPFLK
HVLQLIFRQQTGLVDAITRCYQPFELFDNKTAHLSIPGKSTPEGTLWAHV
LKTVDYGGAEWSLVRIYSGLLKRGDTVRILDTSQSESRQKRQLDETPSCE
VEEIGLLGGRYVYPVHEAHKGQIVLIKGISSAYIKSATLYSVKSKEDMKQ
LKFFKPLDYITEAVFKIVLQPLLPRELPKLLDALNKISKYYPGVIIKVEE
SGEHVILGNGELYMDCLLYDLRASYAKIEIKISDPLTVFSESCSNESFAS
IPVSNISVAAEPMDSKMIQDLSRPRKLSKILRTEYGWDSLASRNVWSFYV
LINDTLPDEISPELLSKYKEQIIQGFYWAVKEGPLAEEPIYGVQYKLLSI
SVPSDVNIDVMKSQIIPLMKKACYVGLLTAIPILLEPIYEVDITVHAPLL
PIVEELMKKRRGSRIYKTIKVAGTPLLEVRGQVPVIESAGFETDLRLSTN
GLGMCQLYFWHKIWRKVPGDVLDKDAFIPKLKPAPINSLSRDFVMKTRRR
KGISTGGFMSNDGPTLEKYISAEL

3D structure

• PDB: 5mps Structure of a spliceosome remodelled for exon ligation.
• Chain: C
• Resolution: 3.85 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	C	E102 H103 Q108 K110 R160 P162 D163 N167 K173 R176 I185 R401	E30 H31 Q36 K38 R88 P90 D91 N95 K101 R104 I113 R329
BS02	GTP	C	L142 H143 G145 K146 T147 S148 L189 S190 G217 K269 R272 S315 T316 K317	L70 H71 G73 K74 T75 S76 L117 S118 G145 K197 R200 S243 T244 K245

Gene Ontology

Molecular Function

• GO:0003924 GTPase activity
• GO:0005515 protein binding
• GO:0005525 GTP binding
• GO:0030623 U5 snRNA binding

Biological Process

• GO:0000244 spliceosomal tri-snRNP complex assembly
• GO:0000349 generation of catalytic spliceosome for first transesterification step
• GO:0000387 spliceosomal snRNP assembly
• GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
• GO:0000398 mRNA splicing, via spliceosome
• GO:0006397 mRNA processing
• GO:0008380 RNA splicing

Cellular Component

• GO:0005634 nucleus
• GO:0005681 spliceosomal complex
• GO:0005682 U5 snRNP
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0046540 U4/U6 x U5 tri-snRNP complex
• GO:0071007 U2-type catalytic step 2 spliceosome

External links

• PDB: RCSB:5mps, PDBe:5mps, PDBj:5mps
• PDBsum: 5mps
• PubMed: 28076345
• UniProt: P36048|SN114_YEAST Pre-mRNA-splicing factor SNU114 (Gene Name=SNU114)