Structure of PDB 5jpi Chain C

Receptor sequence

>5jpiC (length=492) Species: 5807 (Cryptosporidium parvum)

YKMESRIKDISLAEFGLQDMEIAKTDMMGLVELQRKYRDSKPLKGARITG
SLHLTIETSVLVETLYELGAEIRWCSCNIYSTQDHAAAALVKKNIATVFA
WKNETIEDYWVCLNDAMTWRNPDKICGPNLIVDDGGDATLILHEGVKAEI
EYEKYNKIPEYLETELDENGKQLSMDLKCMYKVLKMELLKNPFRWRGMLK
DLYGVSEETTTGVLRLKIMESEGKLLLPAINVNDSVTKSKFDNTYGCRQS
LLHGLFNGCIQMLAGKKIVVLGYGEVGKGCAQGLSGVGARVIVTEIDPIC
ALQASMEGYQVSVLEDVVSEADIFITATGNKDVITVEHMRKMKENAYIAN
IGHFDDEIDVYGLENYPGIKVIEVKQNVHKFTFPDTQKSVILLCKGRLVN
LGCATGHPPLVMSMSFTNQVLAQMDLWKSRELVDRSKNTRFFVKKLSKEL
DEYVARLHLDVLGIKLTKLTETQAKYINVSINGPYKSEDYRY

3D structure

• PDB: 5jpi 2.15 Angstrom Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with D-Eritadenine and NAD.
• Chain: C
• Resolution: 2.15 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H53 S76 S81 D137 E211 N236 K241 D245 N246 C250 H356 H410 S418 Q422
• Catalytic site (residue number reindexed from 1): H53 S76 S81 D134 E208 N233 K238 D242 N243 C247 H353 H407 S415 Q419
• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	C	T212 T213 T214 N246 G275 G277 E278 V279 E298 I299 T331 N333 I354 G355 H356 N403	T209 T210 T211 N243 G272 G274 E275 V276 E295 I296 T328 N330 I351 G352 H353 N400
BS02	DEA	C	H53 T55 E57 E211 T212 K241 D245 L404 G409 H410 M415	H53 T55 E57 E208 T209 K238 D242 L401 G406 H407 M412
BS03	NAD	C	K489 Y493	K486 Y490

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5jpi, PDBe:5jpi, PDBj:5jpi
• PDBsum: 5jpi
• UniProt: Q5CPH1