Structure of PDB 5j33 Chain C

Receptor sequence
>5j33C (length=359) Species: 3702 (Arabidopsis thaliana) [Search protein sequence]
KRYTITLLPGDGIGPEVVSIAKNVLQQAGSLEGVEFNFREMPIGGAALDL
VGVPLPEETISAAKESDAVLLGAIGGYKWDNNEKHLRPEKGLLQIRAALK
VFANLRPATVLPQLVDASTLKREVAEGVDLMVVRELTGGIYFGEPRGIKT
NENGEEVGFNTEVYAAHEIDRIARVAFETARKRRGKLCSVDKANVLEASI
LWRKRVTALASEYPDVELSHMYVDNAAMQLVRDPKQFDTIVTNNIFGDIL
SDEASMITGSIGMLPSASLSDSGPGLFEPIHGSAPDIAGQDKANPLATIL
SAAMLLKYGLGEEKAAKRIEDAVLVALNNGFRTGDIYSAGTKLVGCKEMG
EEVLKSVDS
3D structure
PDB5j33 Structure and Mechanism of Isopropylmalate Dehydrogenase from Arabidopsis thaliana: INSIGHTS ON LEUCINE AND ALIPHATIC GLUCOSINOLATE BIOSYNTHESIS.
ChainC
Resolution3.492 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Y181 K232 D264 D288 D292
Catalytic site (residue number reindexed from 1) Y141 K192 D224 D248 D252
Enzyme Commision number 1.1.1.85: 3-isopropylmalate dehydrogenase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NAD C A113 I114 E129 L132 H321 G322 S323 A324 D326 I327 A333 N334 A73 I74 E89 L92 H281 G282 S283 A284 D286 I287 A293 N294
BS02 MG C D288 D292 D248 D252
BS03 NAD C Y262 N265 Y222 N225
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003862 3-isopropylmalate dehydrogenase activity
GO:0016491 oxidoreductase activity
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
GO:0051287 NAD binding
GO:0070403 NAD+ binding
Biological Process
GO:0009098 L-leucine biosynthetic process
GO:0009553 embryo sac development
GO:0009555 pollen development
Cellular Component
GO:0005829 cytosol
GO:0009507 chloroplast
GO:0009536 plastid
GO:0009570 chloroplast stroma
GO:0009941 chloroplast envelope

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5j33, PDBe:5j33, PDBj:5j33
PDBsum5j33
PubMed27137927
UniProtP93832|LEU32_ARATH 3-isopropylmalate dehydrogenase 2, chloroplastic (Gene Name=IMDH2)

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