Structure of PDB 5hm8 Chain C

Receptor sequence

>5hm8C (length=488) Species: 353152 (Cryptosporidium parvum Iowa II)

KMESRIKDISLAEFGLQDMEIAKTDMMGLVELQRKYRDSKPLKGARITGS
LHLTIETSVLVETLYELGAEIRWCSCNIYSTQDHAAAALVKKNIATVFAW
KNETIEDYWVCLNDAMTWRNICGPNLIVDDGGDATLILHEGVKAEIEYEK
YNKIPEYLETELDENGKQLSMDLKCMYKVLKMELLKNPFRWRGMLKDLYG
VSEETTTGVLRLKIMESEGKLLLPAINVNDSVTKSKFDNTYGCRQSLLHG
LFNGCIQMLAGKKIVVLGYGEVGKGCAQGLSGVGARVIVTEIDPICALQA
SMEGYQVSVLEDVVSEADIFITATGNKDVITVEHMRKMKENAYIANIGHF
DDEIDVYGLENYPGIKVIEVKQNVHKFTFPDTQKSVILLCKGRLVNLGCA
TGHPPLVMSMSFTNQVLAQMDLWKSRELVDRSKNTRFFVKKLSKELDEYV
ARLHLDVLGIKLTKLTETQAKYINVSINGPYKSEDYRY

3D structure

• PDB: 5hm8 2.85 Angstrom Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with Adenosine and NAD.
• Chain: C
• Resolution: 2.85 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H53 S76 S81 D137 E211 N236 K241 D245 N246 C250 H356 H410 S418 Q422
• Catalytic site (residue number reindexed from 1): H52 S75 S80 D130 E204 N229 K234 D238 N239 C243 H349 H403 S411 Q415
• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADN	C	H53 T55 D137 E211 T212 K241 D245 H356 H410 M415	H52 T54 D130 E204 T205 K234 D238 H349 H403 M408
BS02	NAD	C	T212 T213 T214 N246 G277 E278 V279 E298 I299 T331 N333 I354 G355 H356 N403 H410	T205 T206 T207 N239 G270 E271 V272 E291 I292 T324 N326 I347 G348 H349 N396 H403
BS03	NAD	C	K489 Y493	K482 Y486

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5hm8, PDBe:5hm8, PDBj:5hm8
• PDBsum: 5hm8
• UniProt: Q5CPH1