Structure of PDB 5gwe Chain C

Receptor sequence

>5gweC (length=405) Species: 196627 (Corynebacterium glutamicum ATCC 13032)

TSHHGYQPFDMHNPFPAYKELRQEEPVMFDERIGYWVVTKYDDIKTTFDD
WETFSSENAQAPVRKRGPQATQIMTDGGFTAYSGLSARIPPEHTRIRAIA
QKAFTPRRYKALEPDIRAMVIDRVEKMLANDQHVGDMVSDLAYDIPTITI
LTLIGADISMVDTYKRWSDSRAAMTWGDLSDEEQIPHAHNLVEYWQECQR
MVADAHAHGGDNLTADLVRAQQEGQEITDHEIASLLYSLLFAGHETTTTL
ISNCFRVLLDHPEQWQAILENPKLIPAAVDEVLRYSGSIVGWRRKALKDT
EIGGVAIKEGDGVLLLMGSANRDEARFENGEEFDISRANAREHLSFGFGI
HYCLGNMLAKLQAKICLEEVTRLVPSLHLVADKAIGFRENLSFRVPTSVP
VTWNA

3D structure

• PDB: 5gwe Selective oxidation of aliphatic C-H bonds in alkylphenols by a chemomimetic biocatalytic system
• Chain: C
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): A223 A292 E295 T296 T297 C403 L404 G405 Q412 F443
• Catalytic site (residue number reindexed from 1): A173 A242 E245 T246 T247 C353 L354 G355 Q362 F393

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	C	F98 L135 S136 H143 R147 F154 A292 G293 T296 L300 W342 R344 S395 F396 G397 H401 C403 L404 G405 A409	F48 L85 S86 H93 R97 F104 A242 G243 T246 L250 W292 R294 S345 F346 G347 H351 C353 L354 G355 A359
BS02	GWM	C	Q110 S133 G134 L135 S136 R221 S288 F291 A292 W342	Q60 S83 G84 L85 S86 R171 S238 F241 A242 W292	MOAD: Kd=62uM

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0005506 iron ion binding
• GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
• GO:0020037 heme binding
• GO:0046872 metal ion binding

External links

• PDB: RCSB:5gwe, PDBe:5gwe, PDBj:5gwe
• PDBsum: 5gwe
• PubMed: 28607077
• UniProt: Q8NSW2