Structure of PDB 5gsn Chain C

Receptor sequence

>5gsnC (length=445) Species: 89187 (Roseovarius nubinhibens ISM)

TKRVAVIGAGPSGLAQLRAFQSAADQGAEIPEIVCFEKQANWGGLWNYTW
RTGLDENGEPVHCSMYRYLWSNGPKEGLEFADYSFEEHFGKQIASYPPRA
VLFDYIEGRVHKADVRKWIRFNSPVRWVSYDAETAKFTVTAHNHETDSTY
SAAFDHVICASGHFSTPNVPFYEGFDTFNGRIVHAHDFRDAREFEGKDVL
VMGASSSAEDIGSQCWKYGAKSITSCYRSAPMGYAWPDNWEEKPALEKLT
GKTAHFADGSTRDVDAIILCTGYKHFFSFLPDDLRLKTANRLATADLYKG
VAYVHNPAMFYLGMQDQWFTFNMFDAQAWWVRDAILGRITLPKDKAAMLA
DVAERETREEASDDVKYAIRYQADYVKELVAETDYPSFDIDGACDAFFEW
KKHKAKDIMAFRDNSYKSVITGTMAPVHHTPWKEALDDSMEAYLQ

3D structure

• PDB: 5gsn Structural mechanism for bacterial oxidation of oceanic trimethylamine into trimethylamine N-oxide
• Chain: C
• Resolution: 2.203 Å

Enzymatic activity

• Enzyme Commision number: 1.14.13.148: trimethylamine monooxygenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAP	C	N73 F165 P171 Y173 A205 S206 S207 S208 R229 S230 C271 T272 D317 Q318	N72 F164 P170 Y172 A204 S205 S206 S207 R228 S229 C270 T271 D316 Q317
BS02	FAD	C	G9 G11 P12 S13 F37 E38 K39 Q40 G45 L46 W47 H63 S72 N73 P125 V126 A161 S162 G163 F165 F280 Q318 T321 F325	G8 G10 P11 S12 F36 E37 K38 Q39 G44 L45 W46 H62 S71 N72 P124 V125 A160 S161 G162 F164 F279 Q317 T320 F324
BS03	MMZ	C	N73 S208	N72 S207

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0004499 N,N-dimethylaniline monooxygenase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0050661 NADP binding

External links

• PDB: RCSB:5gsn, PDBe:5gsn, PDBj:5gsn
• PDBsum: 5gsn
• PubMed: 27997715
• UniProt: A3SLM3|TMM_ROSNI Trimethylamine monooxygenase (Gene Name=tmm)