Structure of PDB 5f9h Chain C

Receptor sequence

>5f9hC (length=647) Species: 9606 (Homo sapiens)

FKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQK
GKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVEN
NDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNY
LDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIA
TVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRIC
KDREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYN
DALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELES
VSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEG
NPKLSFLKPGILTGRGKASGDHNILIATSVADEGIDIAQCNLVILYEYVG
NVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQ
TWDEAVFREKILHIQTHEKFIRDSVPDKENKKLLCRKCKALACYTADVRV
IEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGI
HVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAE

3D structure

• PDB: 5f9h Structural basis for m7G recognition and 2'-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I.
• Chain: C
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	C	Q299 I300 G326 T347 Q349 K379 Q380 H381 Q507 E510 Q511 R546 K635 T636 R637 G663 T697 S698 V718 N720 K888 K907	Q59 I60 G86 T107 Q109 K139 Q140 H141 Q258 E261 Q262 R297 K386 T387 R388 G414 T428 S429 V449 N451 K613 K632	PDBbind-CN: Kd=1.8nM
BS02	ZN	C	C810 C813 C864 C869	C535 C538 C589 C594
BS03	GTP	C	H830 H847 F853 K858 K861 I875 K888	H555 H572 F578 K583 K586 I600 K613

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:5f9h, PDBe:5f9h, PDBj:5f9h
• PDBsum: 5f9h
• PubMed: 26733676
• UniProt: O95786|RIGI_HUMAN Antiviral innate immune response receptor RIG-I (Gene Name=RIGI)