Structure of PDB 5f98 Chain C

Receptor sequence

>5f98C (length=648) Species: 9606 (Homo sapiens)

PFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQ
KGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVE
NNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFN
YLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVI
ATVKHNLEELEQVVYKPQKFFRKVESRIDKFKYIIAQLMRDTESLAKRIC
KDLREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKY
NDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELE
SVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIE
GNPKLSFLKPGILTGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKM
IQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEA
VFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVR
VIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWG
IHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAE

3D structure

• PDB: 5f98 Structural basis for m7G recognition and 2'-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I.
• Chain: C
• Resolution: 3.28 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	C	N298 Q299 I300 S325 G326 T347 Q349 K379 Q380 H381 Q507 E510 Q511 R546 K635 T636 R637 T662 T697 S698 N720 H830 F853 K858 I875 K888 S906 K909	N59 Q60 I61 S86 G87 T108 Q110 K140 Q141 H142 Q259 E262 Q263 R298 K387 T388 R389 T414 T423 S424 N446 H556 F579 K584 I601 K614 S632 K635	PDBbind-CN: Kd=1.7nM
BS02	ZN	C	C810 C813 C864	C536 C539 C590
BS03	M7G	C	H847 K858 K861 I875 K888	H573 K584 K587 I601 K614

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:5f98, PDBe:5f98, PDBj:5f98
• PDBsum: 5f98
• PubMed: 26733676
• UniProt: O95786|RIGI_HUMAN Antiviral innate immune response receptor RIG-I (Gene Name=RIGI)